Single living cell manipulation and identification using microsystems technologies

作     者：Ion Stiharu Anas Alazzam VahéNerguizian Dacian Roman 

作者机构：Concave Research CentreFaculty of Engineering and Computer ScienceConcordia UniversityMontrealCanada Faculty of EngineeringKhalifa UniversityAbu DhabiUnited Arab Emirates Department of Electrical EngineeringÉcole de Technologie SupérieureMontrealCanada 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2015年第1卷第1期

页      面：35-42页

核心收录：

学科分类：0808[工学-电气工程] 1002[医学-临床医学] 0809[工学-电子科学与技术（可授工学、理学学位）] 0817[工学-化学工程与技术] 0807[工学-动力工程及工程热物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 100214[医学-肿瘤学] 0811[工学-控制科学与工程] 0702[理学-物理学] 10[医学] 

主　　题：circulating tumor cell separation dielectrophoresis identification of circulating tumor cells microsystems RF response spectrum of single living cell 

摘      要：The paper presents the principles and the results of the implementation of dielectrophoresis for separation and identification of rare cells such as circulation tumor cells(CTCs)from diluted blood specimens in media and further label-free identification of the origins of separated cells using radio-frequency(RF)*** separation and the identification units use same fabrication methods which enable system integration on the same *** designs use the advantage of higher surface volume ratio which represents the particular feature for micro-and *** blood in solution of sucrose–dextrose 1–10 is used for cell separation that yields more than 95.3% *** enhanced sensitivity in identification,RF imaging is performed in 3.5–1 solution of glycerol and *** cavity performance method is used to determine the constant permittivity of the cell *** results illustrated by the signature of specific cells subjected to RF imaging suggest a reliable label-free single cell detection method for identification of the type of CTC.