Random-access imaging of awake behaving animals
作者机构:School of Electrical and Computer Engineering and Department of BiologyPurdue UniversityWest LafayetteIN 47907USA
出 版 物:《Light(Science & Applications)》 (光(科学与应用)(英文版))
年 卷 期:2017年第6卷第1期
页 面:666-667页
核心收录:
学科分类:0809[工学-电子科学与技术(可授工学、理学学位)] 08[工学]
基 金:NINDS NIH HHS [U01 NS094341] Funding Source: Medline
摘 要:A compact random-access microscopy system offers inertia-free rapid focusing and line scanning,which enables near simultaneous 3D imaging of neural network in awake behaving animals at highspatiotemporal *** understand the function and principles of operation of neural networks,we need to observe the flow of information in the brain at high-spatiotemporal *** the past two decades,laser scanning two-photon fluorescence microscopy(TPM)combined with sensitive calcium indicators have emerged as the powerhouse for in vivo recording of neuronal *** differs from electrophysiology,in that allows noninvasive measurement of activity at excellent spatial resolution,sufficient for resolving fine structures such as dendritic ***,the high throughput of TPM enables the measurement of a large population of neurons within a single recording.