Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability

作     者：Zhang-yue SONG Jing-luan TIAN Wei-zhe FU Lin LI Ling-hong LU Lian ZHOU Zhi-hui SHAN Gui-xiang TANG Hui-xia SHOU 

作者机构：College of Life SciencesZhejiang University Oil Crops Research InstituteChinese Academy of Agricultural Sciences College of Agriculture and BiotechnologyZhejiang University 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2013年第14卷第4期

页      面：289-298页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：Project supported by the Ministry of Agriculture of China(Nos. 2011ZX08004 and 2009ZX08010013) the National Natural Science Foundation of China(No.31172024) 

主　　题：Soybean Agrobacterium-mediated transformation Genotypes Stable transgenic efficiency 

摘      要：The Agrobacterium-mediated transformation system is the most commonly used method in soybean *** of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation *** this study,twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection,regeneration capacity,and stable transgenic *** genotypes,Yuechun 04-5,Yuechun 03-3,and Tianlong 1,showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental *** the Tianlong 1,the average stable transformation efficiency is 4.59%,higher than that of control genotypes(Jack and Williams 82),which is enough for further genomic research and genetic *** polymerase chain reaction(PCR),LibertyLink strips,and β-glucuronidase(GUS) staining assays were used to detect the insertion and expression of the transgene,leaves painted with 135 mg/L Basta could efficiently identify the transformants.