Effects of blocking Le^Y oligosaccharide on cell surface to MMPs secreted by blastocysts and epithelial cells in mouse in vitro

作     者：Xiaoqi Wang Yu Liu Enkui Duan Guoqing Zeng Zhengmei Zhu 

作者机构：Chinese Acad Sci Inst Zool State Key Lab Reprod Biol Beijing 100080 Peoples R China Dalian Med Univ Dept Biochem Dalian 116027 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：1998年第43卷第17期

页      面：1461-1465页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071003[理学-生理学] 

基　　金：ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (GrantNo .39470 170 )andStateKeyLaboratoryofReproductiveBiology  InstituteofZoology  theChineseAcademyofSciences 

主　　题：mouse Le+Y oligosaccharide antigen matrix metalloproteinases implantation monoclonal antibody. 

摘      要：In order to understand the role of Le+Y oligosaccharide antigen (Le+Y) during implantation, the relationship of Le+Y on the cell surface with matrix metalloproteinase (MMPs) secreted by blastocysts and monolayer epithelial cells during implantation in the mouse %in vitro% was studied by monoclonal antibody (mAb) AH-6, directed to Le+Y[Fuc α1-2 Gal β1-4 (Fuc α1-3) GlcNAc-], and gelatin zymography. The results showed that MMPs secretion was reduced after Le+Y on the cell surface of either epithelial cells or trophoblasts was blocked. It indicated that MMPs expression which played an important function during the process of implantation were regulated by Le+Y. Therefore, it was considered that Le+Y could regulate embryos invasion by some mechanism.