Microfluidics-enabled phenotyping of a whole population of *** worms over their embryonic and post-embryonic development at single-organism resolution

作     者：Maria Cristina Letizia Matteo Cornaglia Raphaël Trouillon Vincenzo Sorrentino Laurent Mouchiroud Maroun S.Bou Sleiman Johan Auwerx Martin A.M.Gijs 

作者机构：Ecole Polytechnique Fédérale de LausanneLaboratory of MicrosystemsLausanneSwitzerland Ecole Polytechnique Fédérale de LausanneLaboratory of Integrative Systems PhysiologyLausanneSwitzerland 

出 版 物：《Microsystems & Nanoengineering》 (微系统与纳米工程（英文）)

年 卷 期：2018年第4卷第1期

页      面：345-355页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：This work in the M.A.G.laboratory was supported by the Ecole Polytechnique Fédérale de Lausanne and the EU Ideas program(ERC-2012-AdG-320404) V.S.was supported by the‘EPFL Fellows’program co-funded by Marie Skłodowska-Curie,Horizon 2020 Grant agreement(665667) M.C.and L.M.were supported by the Gebert Rüf Stiftung(GRS-025/16) .The work in the J.A.laboratory is supported by the EPFL,NIH(R01AG043930) Systems X(SySX.ch 2013/153) Velux Stiftung(1019) the Swiss National Science Foundation(31003A-140780) 

主　　题：fluid treatment otherwise 

摘      要：The organism Caenorhabditis elegans is a performant model system for studying human biological processes and diseases,but until now all phenome data are produced as population-averaged *** of individual responses to drug treatments would however be more ***,a new strategy to track different phenotypic traits of individual *** nematodes throughout their full life-cycle-i.e.,embryonic and post-embryonic development,until adulthood onset,differently from life-span-is *** an automated fashion,single worms were synchronized,isolated,and cultured from egg to adulthood in a microfluidic device,where their identity was preserved during their whole *** phenotypes were monitored and quantified for each animal,resulting in high-content phenome ***,the method was validated by analyzing the response of *** to doxycycline,an antibiotic fairly well-known to prolong the development and activate mitochondrial stress-response pathways in different ***,the obtained extensive single-worm phenome not only confirmed the dramatic doxycycline effect on the worm developmental delay,but more importantly revealed subtle yet severe treatment-dependent phenotypes that are representative of minority subgroups and would have otherwise stayed hidden in an averaged *** heterogeneous response started during the embryonic development,which makes essential having a dedicated chip that allows including this early developmental stage in the drug *** approach would therefore allow elucidating pharmaceutical or therapeutic responses that so far were still being overlooked.