Ozone action on Streptococcus mutans and Lactobacillus fermentum : A pilot study

作     者：Joana Marques Anabela Paula Teresa Gon?alves Manuel Ferreira Eunice Carrilho 

作者机构：Faculty of Medicine the University of Coimbra Area of Dentistry Av Bissaya Barreto Blocos de Celas 3000-075 Coimbra Portugal 

出 版 物：《World Journal of Stomatology》 (世界口腔医学杂志)

年 卷 期：2013年第2卷第1期

页      面：18-23页

学科分类：10[医学] 

基　　金：Supported by GAPI Cabinet Support for Research Projects of Faculty of Medicine of the University of Coimbra process No.19 

主　　题：Dental caries Cariogenic agents Lactobaccilus fermentum Streptococcus mutans Ozone 

摘      要：AIM: To study the effectiveness of ozone in the elimination of cariogenic bacteria, followed with fluoride ***: Sixty extracted teeth free of caries were used, and five groups were constituted. In Group Ⅰ,the teeth were immersed in artificial saliva. In Group Ⅱ,the teeth were inoculated with Streptococcus mutans(S. mutans) and immersed in artificial saliva. In GroupⅢ the teeth were inoculated with Lactobaccilus fermentum(L. fermentum) and immersed in artificial *** Group Ⅳ the teeth were inoculated with S. mutans and L. fermentum and immersed in artificial saliva and the teeth in Group Ⅴ were inoculated with S. mutans and L. fermentum, and were subjected to the application of ozone and to the action of a fluoride mineralizing gel. DIAGNOdent was used to evaluate the caries of the teeth 3 wk after inoculation of bacteria and after that the teeth of Group Ⅴ were subjected to the application of ozone during 60 s, by HealO zone. After the application of ozone, products of the remineralization kit supplied by the manufacturer were applied daily, during 30 d. At the end samples were collected for analysis and evaluation of bacterial activity by polymerase chain reaction. RESULTS: Regarding the value of caries, obtained via DIAGNOdent, in the initial measurement the groups are homogeneous(P = 0.730). There was an increase in DIAGNOdent values, presenting statistical significant difference regarding the initial measurement in all groups(P 0.001), except in group Ⅰ- only artificial saliva- which shows that the artificial carie model was effective. Comparing the initial and final measurements for each of the 60 teeth, it can be observed that in 9 teeth(15.0%) there was a decrease in values between the two measurements, one(1.7%) retained the same values in the two measurements and in the remaining 50 cases(83.3%) there was increase in values between the initial and final measurements. It should also be noted that in the teeth inoculated with S. mutans