Adiponectin affects the mechanical responses in strips from the mouse gastric fundus

作     者：Eglantina Idrizaj Rachele Garella Giovanni Castellini Hermine Mohr Natalia S Pellegata Fabio Francini Valdo Ricca Roberta Squecco Maria Caterina Baccari 

作者机构：Department of Experimental and Clinical Medicine Section of Physiological Sciences University of Florence Psychiatry Unit Department of Health Sciences University of Florence Institute for Diabetes and Cancer Helmholtz Zentrum München 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2018年第24卷第35期

页      面：4028-4035页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：the Florence University(No.RTD CO 090101010107 RICATEN14) Fondazione CRF(No.2017.0777) 

主　　题：Adiponectin Adiponectin receptors Gastric motility Nitric oxide Non-adrenergic,non-cholinergic neurotransmission 

摘      要：AIM To investigate whether the adipocytes derived hormone adiponectin(ADPN) affects the mechanical responses in strips from the mouse gastric fundus. METHODS For functional experiments, gastric strips from the fundal region were cut in the direction of the longitudinal muscle layer and placed in organ baths containing KrebsHenseleit solution. Mechanical responses were recorded via force-displacement transducers, which were coupled to a polygraph for continuous recording of isometric tension. Electrical field stimulation(EFS) was applied via two platinum wire rings through which the preparation was threaded. The effects of ADPN were investigated on the neurally-induced contractile and relaxant responses elicited by EFS. The expression of ADPN receptors, Adipo-R1 and Adipo-R2, was also evaluated by touchdown-PCR analysis. RESULTS In the functional experiments, EFS(4-16 Hz) elicited tetrodotoxin(TTX)-sensitive contractile responses. Addition of ADPN to the bath medium caused a reduction in amplitude of the neurally-induced contractile responses(P 0.05). The direct smooth muscle response to methacholine was not influenced by ADPN(P 0.05). In carbachol precontracted strips and in the presence of guanethidine, EFS induced relaxant responses. Addition of ADPN to the bath medium, other than causing a slight and progressive decay of the basal tension, increased the amplitude of the neurally-induced relaxant responses(P 0.05). Touchdown-PCR analysis revealed the expression of both Adipo-R1 and Adipo-R2 in the gastric *** The results indicate for the first time that ADPN is able to influence the mechanical responses in strips from the mouse gastric fundus.