Cloning of a microphthalmia-associated transcription factor gene and its functional analysis in nacre formation and melanin synthesis in Hyriopsis cumingii
作者机构：Key Laboratory of Genetic Resources for Freshwater Aquaculture and FisheriesMinistry of AgricultureShanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of AquacultureShanghai Ocean UniversityShanghai 201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and BreedingShanghai 201306China
出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)
年 卷 期：2018年第3卷第6期
页 面：217-224页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Work was supported by the earmarked fund for Modern Agro-industry Technology Research System(CARS-49) the National Natural Science Foundation of China(31672654) the Project of Shanghai Engineering and Technology Center for Promoting Ability(16DZ2281200)
主 题：Microphthalmia-associated transcription factor Freshwater pearl mussel Melanin Nacre formation Shell pigmentation
摘 要：Microphthalmia-associated transcription factor(MITF)is an essential transactivator in melanin *** characterize the role of MITF in the pearl mussel Hyriopsis cumingii,the MITF homolog of *** was *** full-length HcMitf cDNA consisted of a 1332-bp with an open reading frame that encode for a 443 amino acid protein that contain a conserved basic helix-loop-helix zipper *** HcMitf was found to widespread tissue distribution but expression was higher in purple mussels than in white mussels,mostly in mantle,liver,kidney,gill,and foot with the exception of the adductor *** and its downstream gene tyrosinase(HcTyr)were highly expressed at the nacre deposition stage after implantation of mantle tissue to produce *** RNA interference,the expression of HcMitf was reduced by 78%(P0.01)and expression of HcTyr was also significantly suppressed and consequently total melanin content was decreased(P0.05).The results suggest that HcMitf plays an important role in melanin synthesis,nacre formation and shell pigmentation in the ***.
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