Photosensitizer Screeningfrom Chinese Medicinal Herbs and Photodynamic Anticancer Studies

作     者：廖静 李萍萍 吴纯静 鄂征 王淑珍 李淑莲 

作者机构：Capital University of Medical Sciences Beijing Beijing Institute for Cancer Research Beijing Norman Bethune University of Medical Sciences Beijing 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：1999年第5卷第3期

页      面：185-189页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：photodynamic anticancer Chinese medicinal herbs human stomach cancer cells photosensitizer screening 

摘      要：Objective: To find new photosensitizers from Chinese medicinal herbs for cancer photodynamic therapy. Methods: The extracts of thirteen herbs were examined: (1) Their fluorescence excitation wave lengths and emission wave lengths; (2) Their fluorescence intensity in living cells and (3) Their distribution and localization in the living cells and the fixed cells both stained in each extract, and responses of cell fluorescence intensity to pH value change. Furthermore, the herb s anticancer photosensitive efficiencies were studied by using BGC823 human stomach cancer cells. Results: Cortex Phellodendri and Rhizoma Coptidis, were found with optimal fluorescence properties as photosensitizers in this test. The latter could remarkably reduce the cell metabolic viability, proliferative ability and increase the cell mortality when the cells exposed to both drugs and luminance but not to drug only. Conclusions: The potential of Chinese medicine as a new kind of photosensitizer and its possibility for using in anticancer photodynamic therapy are existed.