Genetic analysis of polymerase complex(PA,PB1 and PB2) genes of H9N2 avian influenza viruses from Iran(1999 to 2009)

作     者：Masoud Soltanialvar Reza Goodarzi Farshad Akbarnejad 

作者机构：Department of Avian DiseasesFaculty of AgricultureIslamic Azad University Department of Avian PathologyIslamic Azad University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2012年第2卷第11期

页      面：858-862页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by The Islamic Azad University Shoushtar Branch(grantNo.8254) 

主　　题：Genetic analysis Polymerase complex Avian influenza Iran 

摘      要：Objective:To determine the molecular characterization of Polymerase complex(PA,PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relalionsliip of Iranian H9N2 viruses and other Asian ***:The Polymerase complex(PA,PBl and PB2) genes from seven isolates of H9N2 viruses isolated from commercial chickens in Iran during 2008-2009 were amplified(by RT-PCR method) and *** sequences(Open Reading Frame: orf) of the PA,PBl and PB2 genes were used for phylogenetic tree ***:Most PB2 and PA genes of the H9N2 viruses isolated in 2008-2009 belonged to the unknown avian sublineage which grouped with the 2004 Pakistani H7N3 *** PBl genes of Iranian viruses indicated greater genetic diversity and shared a high level of similarity to PBl genes from either HS or H7 subtypes with compared to established H9N2 Eurasian ***:Our findings demonstrated that the H9N2 viruses in Iran exhibit striking reassortment which has led to the generation of new genotypes.