Phosphatidylserine-positive particles in the apical domain of sensory hair cells

作     者：Alfred Nuttall 

作者机构：Oregon Hearing Research CenterOregon Health & Science University3181 SW Sam Jackson Park RoadPortlandOR 97239USA Kresge Hearing Research InstituteThe University of Michigan1301 East Ann StreetAnn ArborMichigan 48109-0506USA 

出 版 物：《Journal of Otology》 (中华耳科学杂志（英文版）)

年 卷 期：2006年第1卷第2期

页      面：76-82页

学科分类：1003[医学-口腔医学] 1002[医学-临床医学] 100213[医学-耳鼻咽喉科学] 10[医学] 

主　　题：PS, Hair cell Annexin V PS-positive particles 

摘      要：Apical membrane recycling has been proposed to be important for normal hair cell function. The current study reports an in vitro work that demonstrates the presence of phosphatidylserine (PS) and PS-positive vesicles labeled by Annexin V in the apical portion of hair cells. The following characteristics of the PS-positive vesicles were noticed using scanning confocal fluorescence microscopy:(1) variable sizes around 200 nm; (2) variable distribution patterns (either uniformly along individual stereocilia in the hair bundle or irregular) in the stereocilia from cell to cell; (3) variable sizes and numbers at locations along the border of the cuticular plate (CP), with a large number of them located at the vestigal kinocilial location; (4) motility with some of the vesicles during the observation period; (5) increase in PS labeling and the number of PS-positive vesicles after loud sound stimulation; and (6) decreased PS labeling and PS-positive vesicle numbers following treatment with LY-294002, a PI3 -kinase inhibitor. These results suggest that the presence of PS-positive vesicles at the apical area of hair cells may be indicative of vesicle shedding or transportation of a protein or rafts.