Map-based cloning of a recessive gene v_1 for virescent leaf expression in cotton(Gossypium spp.)

作     者：ZHANG Youping WANG Qiaolian ZUO Dongyun CHENG Hailiang LIU Ke ASHRAF Javaria LI Simin FENG Xiaoxu YU John Z. SONG Guoli 

作者机构：State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural Sciences U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS)Southern Plains Agricultural Research Center College Station 

出 版 物：《Journal of Cotton Research》 (棉花研究（英文）)

年 卷 期：2018年第1卷第2期

页      面：35-43页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1203[管理学-农林经济管理] 09[农学] 0903[农学-农业资源与环境] 0901[农学-作物学] 0836[工学-生物工程] 0713[理学-生态学] 

基　　金：The National Key Research and Development Program of China(2016YFD0101401) 

主　　题：Cotton Virescence T582 VIGS 

摘      要：Background： Virescence, as a recognizable phenotype available for research on chloroplast development and in the early development stage of cotton, is not only photosynthesis but also for heterosis exploitation in cotton Methods： In current study, for fine mapping of virescent-1 （V1） in cotton, three populations with a total of 5 678 individuals were constructed using T582 which has the virescent trait. Tobacco rattle virus, TRV1 and TRV2 （pYL156）, were used as vectors for the virus-induced gene silencing （VlGS） assay. Results： The V1 gene was fine-mapped to a 20 kb interval on chromosome 20 of tetraploid cotton. We identified only one candidate gene with four single nucleotide polymorphisms between parents, among which the single nucleotide polymorphism at the position of 1 082 base pair caused the change of amino acid residue from Arg （3-79） to Lys （]-582）. The relative expression of the candidate gene in virescent plants was extensively lower than that in normal plants. Nullification of the gene by VlGS significantly turned the green leaf of normal cotton plants into yellow. We named this candidate gene as GhRVL. Conclusions： This study will facilitate the further research on virescent formation, and will be useful for breeding of hybrid cottons.