High-fidelity multimode fibre-based endoscopy for deep brain in vivo imaging

作     者：Sergey Turtaev Ivo T.Leite Tristan Altwegg-Boussac Janelle M.P.Pakan Nathalie L.Rochefort Tomáš Čižmár 

作者机构：Leibniz Institute of Photonic TechnologyAlbert-Einstein-Straße 9Jena 07745Germany School of Life SciencesUniversity of DundeeNethergateDundee DD14HNUK School of Science and EngineeringUniversity of DundeeNethergateDundee DD14HNUK Centre for Discovery Brain SciencesUniversity of EdinburghHugh Robson Building 15George SquareEdinburgh EH89XDUK Center for Behavioral Brain SciencesInstitute of Cognitive Neurology and Dementia ResearchGerman Center for Neurodegenerative DiseasesLeipziger Straße 44Haus 64Magdeburg 39120Germany Simons Initiative for the Developing BrainUniversity of EdinburghEdinburgh EH89XDUK Institute of Scientific Instruments of CASKralovopolska 147Brno 61264Czech Republic 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2018年第7卷第1期

页      面：193-200页

核心收录：

学科分类：080901[工学-物理电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：This work was funded by Marie Curie Actions of the European Union’s FP7 program(608144 to S.T.and I.T.L.,IEF 624461 to J.P.and MC-CIG 631770 to N.R.) the European Regional Development Fund(ERDF:Center for Behavioral Brain Sciences to J.P.and CZ.02.1.01/0.0/0.0/15_003/0000476 to T.Č.) the European Research Council(ERC:724530)to T.Č 

主　　题：multimode fibre vivo 

摘      要：Progress in neuroscience relies on new techniques for investigating the complex dynamics of neuronal *** ongoing challenge is to achieve minimally invasive and high-resolution observations of neuronal activity in vivo inside deep brain *** introduced methods for holographic control of light propagation in complex media enable the use of a hair-thin multimode optical fibre as an ultranarrow imaging *** to endoscopes based on graded-index lenses or fibre bundles,this new approach offers a footprint reduction exceeding an order of magnitude,combined with a significant enhancement in *** designed a compact and high-speed system for fluorescent imaging at the tip of a fibre,achieving a resolution of 1.18±0.04μm across a 50-μm field of view,yielding 7-kilopixel images at a rate of 3.5 frames/***,we demonstrate in vivo observations of cell bodies and processes of inhibitory neurons within deep layers of the visual cortex and hippocampus of anaesthetised *** study paves the way for modern microscopy to be applied deep inside tissues of living animal models while exerting a minimal impact on their structural and functional properties.