Influence of Genista Tinctoria L or Methylparaben on Subchronic Toxicity of Bisphenol A in Rats

作     者：Daniela-Saveta POPA Pompei BOLFA Bela KISS Laurian VLASE Ramona PLTINEAN Anca POP Cornel CTOI Gianina CRISAN Felicia LOGHIN 

作者机构：Department of Toxicology Faculty of Pharmacy Iuliu Hatieganu University of Medicine and Pharmacy Cluj-Napoca Department of Pathology Faculty of Veterinary MedicineUniversity of Agricultural Sciences and Veterinary Medicine Department of Biomedical Sciences Ross University School of Veterinary Medicine Department of Pharmaceutical Technology and Biopharmacy Faculty of Pharmacy ‘Iuliu Hatieganu'University of Medicine and Pharmacy Cluj-Napoca Department of Pharmaceutical Botany Faculty of Pharmacy ‘Iuliu Hatieganu' University of Medicine and Pharmacy Cluj-Napoca 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2014年第27卷第2期

页      面：85-96页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by CNCSIS-UEFISCSU Romania project number PNII-IDEI 1337/2008 

主　　题：Food contaminant Phytoestrogen Morphological effect Lipid peroxidation Hydroxylradical 

摘      要：Objective To evaluate the influence of an extract of Genista tinctoria L. herba （GT） or methylparaben （MP） on histopathological changes and 2 biomarkers of oxidative stress in rats subchronicly exposed to bisphenol A （BPA）. Methods Adult female Wistar rats were orally exposed for 90 d to BPA （50 mg/kg）, BPA＋GT （35 mg isoflavones/kg） or BPA＋MP （250 mg/kg）. Plasma and tissue samples were taken from liver, kidney, thyroid, uterus, ovary, and mammary gland after 30, 60, and 90 d of exposure respectively. Lipid peroxidation and in vivo hydroxyl radical production were evaluated by histological analysis along with malondialdehyde and 2,3-dihydroxybenzoic acid detection. Results The severity of histopathological changes in liver and kidneys was lower after GT treatment than after BPA or BPA＋MP treatment. A minimal thyroid receptor antagonist effect was only observed after BPA＋MP treatment. The abnormal folliculogenesis increased in a time-dependent manner, and the number of corpus luteum decreased. No significant histological alterations were found in the uterus. The mammary gland displayed specific estrogen stimulation changes at all periods. Both MP and GT revealed antioxidant properties reducing lipid peroxidation and BPA-induced hydroxyl radical generation. Conclusion GT L. extract ameliorates the toxic effects of BPA and is proved to have antioxidant potential and antitoxic effect. MP has antioxidant properties, but has either no effect or exacerbates the BPA-induced histopathological changes.