Identification and Characterization of Reduced Epicuticular Wax Mutants in Rice

作     者：Thomas H.TAI 

作者机构：United States Department of Agriculture-Agricultural Research ServiceCrops Pathology and Genetics Research UnitDepartment of Plant SciencesMail Stop 1University of California 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2015年第22卷第4期

页      面：171-179页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 0902[农学-园艺学] 

基　　金：supported by United States Department of Agriculture-Agricultural Research Service Current Research Information System Project 5306-21000021-00D Mars, Inc. Cooperative Research and Development Agreement (No. 58-3K95-2-1563) 

主　　题：chemical mutagenesis cuticle wax forward genetics membrane permeability rice scanning electron microscopy 

摘      要：Epicuticular wax forms the outermost protective barrier of the aerial surfaces of land plants, working in concert with other components of the plant cuticle to prevent uncontrolled loss of water and to provide protection against an array of external environmental stress. In this study, chemically- mutagenized populations of rice （Oryza sativa L.） derived from approximately 4 750 M2 families were screened for adhesion of water droplets resulting in a wet leaf/glossy （wig） phenotype. Mutants were identified in 11 independently-derived M2 families. Scanning electron microscopy analysis confirmed the association of the wig phenotype with changes in the epicuticular wax crystals of these plants. The phenotypes of five mutants （7-17A, 26.1, 524.2, 680.2, and 843.1） were confirmed to be the result of single recessive gene mutation. Evaluation of mutants from 3 （6-1A, 7-17A, and 11-39A） of 11 M2 families revealed significant reductions （〉 50%） in surface wax content and increases in cuticle membrane permeability.