Flow Field-Flow Fractionation with Mass Spectrometry for Top-Down and Bottom-Up Lipidomics
作者机构：Department of ChemistryYonsei UniversitySeoul 03722Korea
出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)
年 卷 期：2017年第1卷第3期
页 面：193-201页
学科分类：0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学]
基 金：Grant from the National Research Foundation of Korea(NRF-2015R1A2A1A01004677)
主 题：Lipids Lipoproteins Flow field-flow fractionation Nanoflow liquid chromatography electrospray-ionization tandem mass spectrometry
摘 要：As lipidomics has attracted increased attention in life science,advanced mass spectrometry(MS)technologies have been combined with other separation techniques to improve and expand the branch of *** review intends to provide general knowledge of offline and online coupling of flow field-flow fractionation(FlFFF)—a technique that encompasses the separation of nano-to micro-scale biomolecules—with MS for analysis of blood plasma lipoproteins,processes that are considered bottomup and top-down approaches,*** first part of this review focuses on the bottom-up method using multiplexed hollow fiber FlFFF(MxHF5)and nanoflow liquid chromatography electrospray-ionization tandem mass spectrometry(nLC-ESI–MS/MS)for non-targeted identification of *** this protocol,plasma lipoproteins of different types are collected using MxHF5,and the lipids within the lipoproteins are then extracted and analyzed via nLC-ESI–MS/*** second part of the review describes the top-down approach,which uses online coupling of miniaturized FlFFF to ESI–MS for a fast screening of targeted ***,the separation of lipoproteins and detection of their component lipids are achieved *** both methods aim to quantify the lipids within lipoproteins,the bottom-up approach provides an extensive lipidome,whereas the top-down method is suitable for high-speed targeted lipidomic *** review discusses variants of FlFFF-ESI–MS/MS that offer effective analytical technologies for lipidomics.
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