Antidiarrheal properties of different extracts of Chinese herbal medicine formula Bao-Xie-Ning

作     者：Jing Li Xin-lin Wu Yuling Chen Zhi Tang Yue-hong Xu Jian-min Jiang Yue-yu Gu Jian-ping Chen De-po Yang Dong-mei Wang 

作者机构：School of Pharmaceutical Sciences Sun Yat-sen University Department of Traditional Chinese Medicine the First Affiliated Hospital Sun Yat-sen University Faculty of Pharmacy University of Sydney Traditional Medicine Chinic Kiang Wu Hospital School of Chinese Medicine University of Hong Kong 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2013年第11卷第2期

页      面：125-134页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported and funded by the State Administration of Traditional Chinese Medicine of China (No.2003LHR20) 

主　　题：drugs, Chinese herbal antidiarrhea pain analgesic and spasmolytic activities 

摘      要：OBJECTIVE： Bao-Xie-Ning （BXN）, a traditional Chinese herbal medicine （CHM） formula composed of Fructus Evodiae, Flos Caryophylli and Cortex Cinnamomi, and used for the treatment of infant diarrheal illness, was subject to systematic assessment for its putative multiple pharmacodynamic effects and pharmacological antidiarrheal mechanisms. METHODS： High-performance liquid chromatography-diode array detector-electrospray ionization- mass spectrometric/mass spectrometry was developed and validated for identification and quantification of the main constituents in different extracts of BXN. Male Kunming mice weighing 20 to 25 g were used for detecting the antidiarrheal activity of the extracts. Ethanolic extract （EE）, volatile oil extract （VOE）, and aqueous extract （AE） of BXN were respectively subjected to pharmacodynamic and pharmacological comparison in assessing antidiarrheal effects with senna-induced diarrhea, castor oil-induced diarrhea, acetic acid-induced writhing assay, and isolated duodenum test. RESULTS： The highest yields of three detected components of BXN, rutaecarpine, eugenol and cinnamaldehyde were observed in EE. EE showed the most remarkable antidiarrheal activity in dose-dependent and time-dependent manners in both senna- and castor oil-induced diarrhea models, and presented dose-dependent analgesic activity in acetic acid-induced algesthesia model. In addition, EE extract of BXN also exhibited strong antimobility action on the intestine and strongest depression on spontaneous contraction of isolated duodenum. CONCLUSION： Ethanol extraction is an efficient method to extract the active constituents of BXN. BXN extract demonstrated multiple pharmacological activities affecting the main mechanisms of diarrhea, which validated BXN＇s usage in the comprehensive clinical treatment of diarrhea.