High incidence of biliary complications in rat liver transplantation:Can we avoid it?
High incidence of biliary complications in rat liver transplantation:Can we avoid it?作者机构：Department of Hepa-tobiliary Surgery Sun Yat-sen Memorial Hospital Sun Yat-senUniversity Haizhu District Guangzhou 510260 GuangdongProvince China Department of Breast Surgery GuangzholWomen and Children＇s Medical Center Guangzhou 510260Guangdong Province China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2011年第17卷第26期
页 面：3140-3144页
核心收录：
学科分类：090603[农学-临床兽医学] 0710[理学-生物学] 07[理学] 09[农学] 0906[农学-兽医学] 071002[理学-动物学]
基 金：Supported by National Natural Science Foundation of China No.30671987
主 题：Rat Liver transplantation Biliary complication Animal model
摘 要：MM： To investigate how to reduce the incidence of biliary complications in rat orthotopic liver transplantation. METHODS： A total of 165 male Wistar rats were ran- domly divided into three groups： Group A, orthotropic liver transplantation with modified ＂two-cuff＂ technique; Group B, bile duct was cut and reconstructed without transplantation; and Group C, only laparotomy was performed. Based on the approaches used for biliary reconstruction, Group A was divided into two sub-groups： A1 （n = 30）, duct-duct reconstruction, and A2 （n = 30）, duct-duodenum reconstruction. To study the influence of artery reconstruction on bile duct complication, Group B was divided into four sub-groups： B1 （n = 10）, duct-duct reconstruction with hepatic artery ligation, B2 （n = 10）, duct-duct reconstruction without hepatic artery ligation, B3 （n = 10）, duct-duodenum reconstruction with hepatic artery ligation, and B4 （n = 10）, duct-duodenum recon- struction without hepatic artery ligation. The samples were harvested 14 d after operation or at the time when significant biliary complication was found. RESULTS： In Group A, the anhepatic phase was 13.7 ＋ 1.06 min, and cold ischemia time was 50.5 ＋ 8.6 min. There was no significant difference between A1 and A2 in the operation duration. The time for biliary reconstruction was almost the same among all groups. The success rate for transplantation was 98.3% （59/60）. Significant differ- ences were found in the incidence of biliary complications in Groups A （41.7%）, B （27.5%） and C （0%）. A2 was more likely to have biliary complications than A1 （50% vs 33.3%）. B3 had the highest incidence of biliary complica- tions in Group B. CONCLUSION： Biliary complications are almost in- evitable using the classical ＂two cuff＂ techniques, and duct-duodenum reconstruction is not an ideal option in rat orthotopic liver transplantation.
维普期刊数据库