Differential expression of Bcl-2 and Bax during gastric ischemia-reperfusion of rats
Differential expression of Bcl-2 and Bax during gastric ischemia-reperfusion of rats作者机构：Department of Physiology Xuzhou Medical College 84 WestHuaihai Road Xuzhou 221002 Jiangsu Province China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2011年第17卷第13期
页 面：1718-1724页
核心收录：
学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 1002[医学-临床医学] 07[理学] 09[农学] 0906[农学-兽医学] 071002[理学-动物学]
基 金：Supported by grants from the National Natural Science Foun-dation of China, No. 30570671 the Natural Science Founda-tion of Jiangsu Province, No. BK2009088 the Natural ScienceFund for Colleges and Universities in Jiangsu Province, No.10KJB310015 the Xuzhou Social Development Fund, No.XM08C062
主 题：Stomach Ischemia-reperfusion Bcl-2 Bax Extracellular signal-regulated kinase 1/2
摘 要：AIM: To investigate expression of Bcl-2 and Bax in gastric ischemia-reperfusion (GI-R) and involvement of extracellular signal-regulated kinase (ERK) 1/2 activation. METHODS: The GI-R model was established by ligature of the celiac artery for 30 min and reperfusion in SpragueDawley rats. Rats were assigned to groups in accordance with their evaluation period: control, 0, 0.5, 1, 3, 6, 24, 48, and 72 h. Expression and distribution of Bcl-2 and Bax proteins were analyzed by immunohistochemistry and western blotting in gastric tissue samples after sacrifice. RESULTS: Compared with controls, the percentage of positive cells and protein levels of Bcl-2 decreased inthe early phases of reperfusion, reached its minimum at 1 h (P 0.05); it then increased, reaching its peak at 24 h of reperfusion (P 0.05). The pattern of Bax expression was opposite to that of Bcl-2. Bax expression increased after reperfusion, with its peak at 1 h of reperfusion (P 0.05), and then it decreased gradually to a minimum at 24 h after reperfusion (P 0.05). On the other hand, inhibition of activation of ERK1/2 induced by PD98059, a specific upstream MEK inhibitor, had significant effects on Bcl-2 and Bax in GI-R. Compared with GI-R treatment only at 3 h of reperfusion, PD98059 reduced the number of Bcl-2 positive cells (0.58% of R3h group, P 0.05) and Bcl-2 protein level (74% of R3h group, P 0.05) but increased the number of Bax-positive cells (1.33-fold vs R3h group, P 0.05) and Bax protein level (1.35-fold of R3h group, P 0.05). CONCLUSION: These results indicated that the Bcl-2 and Bax played a pivotal role in the gastric mucosal I-R injury and repair by activation of ERK1/2.
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