Determination of diastereoisomeric alkaloids in urine by capillary electrophoresis with electrochemiluminescence detection

作     者：Yan Ming Liu Long Fei Peng Lin Mei Li Juan Liu 

作者机构：College of Chemistry and Chemical Engineering Xinyang Normal University Xinyang 464000 China Received 1 July 2010 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2011年第22卷第2期

页      面：197-200页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 070301[理学-无机化学] 

基　　金：the National Natural Science Foundation of China(No.21075106) Henan Innovation Project for University Research Talents(No.2005126) Natural Science Foundation of Henan Province of China (No.092300410122) 

主　　题：CE Electrochemiluminescence Alkaloids Urine analysis Pharmacokinetics 

摘      要：A new and simple capillary electrophoresis with electrochemiluminescence detection was developed for the separation and the quantification of a pair of diastereoisomenc alkaloids（ephedrine and pseudoephedrine）.The limits of detection（S/N = 3） were 4.5×10^-8 mol/L for ephedrine and 5.2×10^-8 mol/L for pseudoephedrine,*** RSDs of migration time and peak area were less than 1.3 and 2.5%（n = 5）,*** applicability of the propose method was illustrated in the determination of ephedrine and pseudoephedrine in human urine,ephedrine in nasal drops,and the monitoring of pharmacokinetics for pseudoephedrine.