Impact of xylanases on gut microbiota of growing pigs fed corn-or wheat-based diets

作     者：Zhengxiao Zhang Hein M.Tun Ru Li Beatriz J.M.Gonzalez Hannah C.Keenes Charles M.Nyachoti Elijah Kiarie Ehsan Khafipour 

作者机构：Department of Animal Science University of Manitoba Department of Medical Microbiology and Infectious Diseases University of Manitoba Department of Animal Bioscience University of Guelph 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2018年第4卷第4期

页      面：339-350页

核心收录：

学科分类：0710[理学-生物学] 090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 

基　　金：supported by grants from Growing Forward 2-Agricultural Rural Development Initiative Program of the Province of Manitoba Canada and DuPont 

主　　题：Piglets Microbiota Xylanase Arabinoxylans Digestibility 

摘      要：This study investigated the effects of xylanase supplementations with cereal-based diets on nutrient digestibility and gut microbiota of growing pigs. A total of 96 individually penned pigs(initial BW = 22.7 ± 0.65 kg) were allotted to 12 treatments and subjected to a completely randomized block design experiment. Pigs in each treatment were fed an isocaloric wheat-based or corn-based diet with or without 1 of 5 types of xylanase supplements(XA, XB, XC, XD, XE). On d 42, all piglets were euthanized to obtain ileal and cecal digesta for microbial analysis, which involved high-throughput sequencing of the V1 - V3 regions of 16 S rRNA gene. Corn-and wheat-based diets differed(P 0.05) in digestion characteristics. Dietary treatments affected the alpha-and beta-diversities of microbiota in the cecum but not in the ileum. The wheat-based diet increased(P 0.05) alphadiversity and clustered separately(P 0.05) compared with the corn-based diet. Wheat-based diet also promoted the relative abundance of genus(g.) Succinivibrio while corn-based diet promoted the proportion of family(f.) Veillonellaceae in the community. Among xylanases, only XC within the wheat-based diet altered(P 0.05) the beta-diversity of the cecal microbiota compared with control. For each cereal-based diet and compared with the controls, xylanase treatments affected(P 0.05) the proportions of 5 bacterial taxa in the ileum(f. Peptostreptococcaceae, order [o.]Streptophyta, f. Clostridiaceae, g. Clostridium and g. Streptococcus) and 8 in the cecum(g. Lactobacillus,g. Streptococcus, class [c.] Clostridia, f. Clostridiaceae, g. Megasphaera, g. Prevotella, g. Roseburia and ***). Network analysis showed that across diets under control treatments, Bacteroidetes was the most influential phylum promoting cooperative relationships among members of the ileum and cecum microbiota. Xylanase treatment, however, reduced the influence of Bacteroidetes and promoted a large number of hub taxa majority