S1B-5 Macrophage Gene Therapy in the Central Nervous System
作者机构：Department of Biomedical SciencesPaul L.Foster School of Medicine Gradulate School of Biomedical SciencesTexas Tech University Health Sciences CenterEl PasoTexas79905USA Weifang Medical UniversityShandongChina
出 版 物：《神经药理学报》 (Acta Neuropharmacologica)
年 卷 期：2018年第8卷第4期
页 面：32-33页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：MDM In LPSinduced inflammatory
摘 要：Regulation of immune responses is particular important in recovery after stroke,traumatic brain injury,and brain infections by inhibiting inflammation and reinstating rescue *** delivery based immunotherapy appears to have significant therapeutic potential for the treatment of neurological conditions by inhibition of neuroinflammation to prevent irreversible destructive ***,current gene delivery systems lack desired convenience,efficiency,and *** offers an attractive gene delivery system that overcome the immunogenic and long-term oncologic effects of the viral *** branched PEI lipids with PLGA,we developed a highly efficient core-shell nanoparticle(NP)gene delivery *** IL-4 plasmid DNA(pIL-4)loaded NPs(pIL-4-NPs)were effectively delivered pIL-4 to the human blood monocytes derived macrophages(MDM)and mouse bone marrow-derived macrophages(BMM).Furthermore,pIL-4-NPs quickly escaped lysosomes and entered the nuclei of these *** in vitro transfection assays,the pIL-4-NPs had higher transfection efficiency,in contrast,PEI25KPEI liposomes and PLGA NPs were unable to transfect MDM and *** LPSinduced inflammatory in vitro and in vivo models,transfection of pIL-4-NPs decreased LPSinduced pro-inflammatory cytokines,such as IL-1,IL6,and TNF-.Our findings indicate that pIL-4-NPs delivery system is capable of effectively deliver IL-4 plasmid DNA into human and murine macrophages,which consistently express IL-4 in vitro and in *** pIL-4-NPs dramatically diminished pro-inflammatory cytokines expression,exhibited antineuroinflammatory and neuroprotective efficacy when macrophages were activated by ***,branched PEI lipids and PLGA formed core-shell NPs are a promising gene delivery platform for immunotherapy in neurological ailments.
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