Changes in the expression of platelet-derived growth factor in astrocytes in diabetic rats with spinal cord injury

作     者：WU Qiong-hua CHEN Wei-shan CHEN Qi-xin WANG Jian-hong ZHANG Xiao-ming 

作者机构：Department of Orthopedics Second Affiliated Hospital ZhejiangUniversity School of Medicine Hangzhou Zhejiang 310009China Department of Anatomy and Cell Biology Zhejiang UniversitySchool of Medicine Hangzhou Zhejiang 310058 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2010年第123卷第12期

页      面：1577-1581页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：This work was supported by a grant from Zhejiang Provincial Natural Science Foundation (No. Y2080348) 

主　　题：platelet-derived growth factor spinal cord injury diabetes mellitus rat 

摘      要：Background Prospective mortality studies in the United States revealed that the mortality was elevated in diabetics compared to normal individuals following chronic spinal cord injury （SCI）. Our study was conducted to investigate the levels of platelet-derived growth factor （PDGF） of astrocytes in SCI in streptozotocin （STZ）-induced diabetic rats. Methods Thirty male Sprague-Dawley （SD） rats were randomly divided into 3 groups： SCI group, diabetic SCI group, and sham operation control group. We employed STZ-induced diabetic SD rats and a weight-drop contusion SCI model. The rats were sacrificed on day 7 after the induction of SCI. Immunohistochemistry and Western blotting analysis were used to detect the PDGF expression level. Basso, Beattie and Bresnahan locomotor rating scale （BBB） was also used to evaluate the neurological recovery level of the rats. Results PDGF positive astrocyte numbers were significantly higher and PDGF staining was more intensive in astrocytes in the SCI group than in the diabetic SCI group （P〈0.05）. The diabetic SCI group showed a slower recovery of motor function with a lower BBB score 7 days after acute spinal injury. Conclusions PDGF is an important factor for the recovery of neurological function after acute spinal injury and hyperglycemia in diabetic rats could depress the expression of PDGF in injured spinal cord. This may help to explain the slower recovery and higher mortality in diabetics after SCI.