Protective effect of electroacupuncture preconditioning at zúsānlǐ(足三里 ST36) on mitochondria in the intestinal ischemia/reperfusion injury

作     者：Zheng HUANG Yong-ming HAN Xiao-ping HONG Yan-jun DUAN Tao CHEN Jiao-rong CHEN 黄征;韩永明;洪小平;段妍君;陈涛;陈姣蓉

作者机构：Department of Anatomy and Histology and Embryology Basic Medical College Hubei University of traditional Chinese medicine Wuhan Hubei 430065 China pathology Department of the Central Hospital of Wuhan Huazhong University of Science and Technology Wuhan Hubei 430014 China 

出 版 物：《World Journal of Acupuncture-Moxibustion》 (世界针灸杂志（英文版）)

年 卷 期：2018年第28卷第3期

页      面：191-197,I0005页

核心收录：

学科分类：1005[医学-中医学] 100512[医学-针灸推拿学] 10[医学] 

基　　金：supported by a grant from the National Natural Science Foundation of Hubei Province of China(Grant no.2017CFB384) 

主　　题：Electroacupuncture ST36 (Zusanli acupoint) Intestinal ischemia/reperfusion Intestinal ischemia/reperfusion Mitochondria Cytochrome c 

摘      要：Objective： The study explored the effect of applying electroacupuncture（EA） preconditioning at ST 36 on mitochondria in rats with intestinal ischemia/reperfusion ***： Forty SD rats were divided into four sets： sham operation group（sham group）; intestinal ischemia/reperfusion group（I/R group）; EA preconditioning at ST 36 followed by intestinal ischemia/reperfusion injury（ST 36 ＋ I/R group）; EA preconditioning at the lateral site away from ST360.5 cm followed by intestinal ischemia/reperfusion injury（N＋I/R group）. For the sham group, the rats were opened abdominal cavity for 3 h and 20 min and their abdominal cavities were covered with wet gauze avoiding drying and kept on the thermostat at 37 0 C. For the ischemia/reperfusion（I/R） group,rats were anaesthetised and their abdominal cavities were opened to expose jejunum segments. The segment＇s collateral blood supply was restricted by bilateral ligation of the intestine. Next, one of the branches of a mesenteric artery was occluded with a thread for 20 min and then the thread was released after such ischemia conditions, keeping reperfusion for 3 h. For the ST36 ＋ I/R group, the electroacupuncture at ST36 was first performed, then the intestinal ischemia/reperfusion model was constructed. For the N ＋ I/R group, electroacupuncture at non ST36 acupoint, which is away from ST36 about 0.5 cm, and then the intestinal ischemia/reperfusion model was performed. Measurements of the levels of inflammatory markers tumour necrosis factor a（TNFa） and interleukin-1 beta（IL-1β）, cytochrome c（CYCS）, and the mitochondrial membrane pro-apoptotic protein（BAX）, anti-apoptotic protein Bcl-2 were ***： Compared to I/R group, the intensity of cytoplasmic CYCS in intestinal tissues was significantly decreased in the ST 36 ＋ I/R group（1.65 vs. 0.18, p〈0.05）. Compared to N ＋ I/R group, the intensity of cytoplasmic CYCS in intestinal tissues was also dramatically declined in the ST 36 ＋ I/R group（1.37 vs. 0.18, p〈0.05）. Th