Pterygial body epithelium domination of pterygial proliferation with TCF4 as a potential key factor

作     者：Cong Nie Xin-Chun Zhang Si-Ying Xu Ya-Dan Quan Zhi-Xin Tang Rong Lu 

作者机构：State Key Laboratory of OphthalmologyZhongshan Ophthalmic CenterSun Yat-sen University Department of ProsthodonticsHospital of StomatologyGuangdong Provincial Key Laboratory of StomatologyGuanghua School of StomatologySun Yat-sen University 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2018年第11卷第9期

页      面：1467-1474页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China(No.81670823) 

主　　题：pterygium epithelium proliferation transcription factor 4 

摘      要：AIM： To characterize the proliferative capacity of pterygial epithelium in different regions（head, neck and body） of pterygium and explore the function of transcription factor 4（TCF4） in pterygium ***： Thirty pterygium tissues and 10 normal conjunctival tissues were obtained from Zhongshan Ophthalmic Center（ZOC） and Guangdong Eye Bank, respectively. Proliferative capacity of head, neck and body in pterygial epithelium was measured using clonal analysis, fold growth analysis and expression profile of proliferative markers revealed by immunofluorescent staining and real-time PCR. The expression of TCF4 was highlighted by double immunofluorescent staining with other proliferation related markers such as proliferating cell nuclear antigen（PCNA） and ATP-binding cassette subfamily G member 2（ABCG2）.RESULTS： The proliferative potential of pterygial epithelium was higher than that of normal conjunctival epithelium. High expression levels of proliferative markers（P63α, PCNA and ABCG2） in pterygial body epithelium were observed in immunofluorescent staining and real-time PCR（P〈0.05）. Also, epithelial cells isolated from pterygial body demonstrated higher proliferative capacity in clonal analysis and fold growth analysis, than those isolated from the head and neck regions. The TCF4 expression in pterygial epithelium was similar to other proliferative markers（P63α, PCNA and ABCG2）, as higher in pterygial body than head and neck. Moreover, TCF4 showed coexpression with other proliferation-related markers（PCNA and ABCG2） in the double immunofluorescent staining ***： The proliferative capacity in pterygial body epithelium is prominent than the head and neck regions, and upregulated TCF4 may be associated with enhanced proliferation in the pterygium.