Carcinogenesis of asbestos switched on by inducing cross-linkage between DNA complementary pair bases

作     者：DAI Qianhuan CHEN Sha LI Yingxia 

作者机构：Center of Environmental SciencePeking UniversityBeijing 100871China Center for Chemistry and Bioengineering of CancerBeijing Polytechnic UniversityBeijing 100022China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2002年第47卷第13期

页      面：1086-1091页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

基　　金：This work was supported by the National Natural Science Foundation of China (Grant No. 20042001) 

主　　题：asbestos mechanism of carcinogenesis di-region theory hydroxyl free radical DNA interstrand cross-link Fenton reaction AM1 molecular orbital calculation. 

摘      要：Since the beginning of the 1980s, Dai Qianhuan predicted based upon his di-region theory that the carcino-genesis switched on by the so-called physical carcinogenic factors including radiation, asbestos and foreign matter implantation, is just initiated through the cross-linking between DNA complementary pair bases induced by them. In this note, it was evidenced with the DNA filter elution method that the oxygenase activated by asbestos induces the cross-linking between DNA inter-strands and DNA-protein with dosage correlation, in which over 80% of DNA inter-strand cross-link ratio account for the total cross-link ratio. Obviously, both of the cross-linkages are just induced by hydroxyl free radical, HO·, because the ferrous ion increased the cross-link ratios up to several times through Fenton reaction and vitamin C inhibited the cross-link ratios with factors of 8-9 by destroying the hydroxyl radical. Non-carcinogen but with lower free radical formation energy, pyrene, by culturing with asbestos