Hepatoprotective effects of cathepsin B inhibitor on acute hepatic failure induced by lipopolysaccharide/D-galactosamine in mice

作     者：Bing-Zhu Yan Li-Yan Chen Lan Kang Xiao-Ren Wang Man-Ru Bi Wei Wang Bao-Shan Yang 

作者机构：Department of Infectious DiseasesSecond Clinical HospitalHarbin Medical University 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2013年第12卷第1期

页      面：80-86页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：acute hepatic failure cathepsin B inhibitor hepatocyte apoptosis caspases cytochrome c 

摘      要：BACKGROUND:Increasing evidence suggests that the inactivation of cathepsin B attenuates hepatocyte apoptosis and liver *** study aimed to investigate the protective effects of a cathepsin B inhibitor(CA-074me) on lipopolysaccharide(LPS)/D-galactosamine(D-GalN)-induced acute hepatic failure(AHF) in ***:Mice were intraperitoneally injected with a combination of LPS/D-GalN to induce AHF with or without CA-074me *** cumulative survival rates were calculated 48 hours after the induction of *** well as changes in biochemical indicators and liver histology,hepatocyte apoptosis was assessed using a TUNEL *** tumor necrosis factor-α(TNF-α) production,caspase-3,caspase-8,and caspase-9 activity was *** cytochrome c and Bcl-2 expression were measured by Western ***:The marked elevation in serum aminotransferase activity and prothrombin time found in LPS/D-GalN-treated mice was significantly improved by pretreatment with *** efficacy of CA-074me was also confirmed by histological analysis and TUNEL *** survival rate significantly improved in LPS/D-GalN-induced mice given CA-074me compared with untreated ***/D-GalNinduced caspase-3 and caspase-9 activation was remarkably suppressed by ***,the increased levels of serum TNF-α and elevated caspase-8 activity in AHF mice were not significantly reduced by ***,CA074me sharply reduced the increased expression of cytosolic cytochrome c and markedly augmented Bcl-2 ***:These results suggest that CA-074me has a protective effect in acute hepatic failure induced by LPS/D-GalN.