Nanoscale chemical imaging of individual chemotherapeutic cytarabineloaded liposomal nanocarriers

作     者：Karin Wieland Georg Ramer Victor U. Weiss Guenter Allmaier Bemhard Lendl Andrea Centrone 

作者机构：Institute of Chemical Technologies and AnalyticsResearch Division EnvironmentalProcess Analytics and SensorsTU WienVienna 1060Austria Center for Nanoscale Science and TechnologyNational Institute of Standards and TechnologyGaithersburgMD 20899USA Institute for Research in Electronics and Applied PhysicsUniversity of MarylandCollege ParkMD 20742USA Institute of Chemical Technologies and AnalyticsResearch Division Instrumental and Imaging Analytical ChemistryTU WienVienna 1060Austria 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2019年第12卷第1期

页      面：197-203页

核心收录：

学科分类：0808[工学-电气工程] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：support by the Austrian Research Funding Association (FFG) within the research project “NanoSpec – Highresolution near-field infrared microscopy for the process control of nanotechnological components” support from the University of Maryland through the Cooperative Research Agreement between the University of Maryland and the National Institute of Standards and Technology Center for Nanoscale Science and Technology 

主　　题：tapping photothermal induced resonance (PTIR) nanoscale chemical imaging liposomes cytarabine drug delivery nanocarriers 

摘      要：Dosage of chemotherapeutic drugs is a tradeoff between efficacy and *** are nanocarriers that increase therapy efficacy and minimize side-effects by delivering otherwise difficult to administer therapeutics with improved efficiency and ***,variabilities in liposome preparation require assessing drug encapsulation efficiency at the single liposome level,an information that,for non-fluorescent therapeutic cargos,is inaccessible due to the minute drug load per *** induced resonance (PTIR) provides nanoscale compositional specificity,up to now,by leveraging an atomic force microscope (AFM) tip contacting the sample to transduce the sample s photothermal ***,on soft samples (e.g.,liposomes) PTIR effectiveness is reduced due to the likelihood of tip-induced sample damage and inefficient AFM ***,individual liposomes loaded with the chemotherapeutic drug cytarabine are deposited intact from suspension via nano-electrospray gas-phase electrophoretic mobility molecular analysis (nES-GEMMA) collection and characterized at the nanoscale with the chemically-sensitive PTIR method.A new tapping-mode PTIR imaging paradigm based on heterodyne detection is shown to be better adapted to measure soft samples,yielding cytarabine distribution in individual liposomes and enabling classification of empty and drug-loaded *** measurements highlight PTIR capability to detect ~ 103 cytarabine molecules (~ 1.7 zmol) label-free and non-destructively.