Prostate cancer tends to metastasize in the bone-mimicking microenvironment via activating NF-κB signaling

作     者：Haibo Tong Chunlin Zou Siyuan Qin Jie Meng Evan T.Keller Jian Zhang Yi Lu 

作者机构：Key Laboratory of Longevity and Aging-related Diseases Guangxi Medical University Ministry of Education Nanning Guangxi 530021 China Southern University of Science and Technology School of Medicine Shenzhen Guangdong 518055 China Department of Pathology and Internal Medicine University of Michigan Ann Arbor MI 48109 USA 

出 版 物：《The Journal of Biomedical Research》 (生物医学研究杂志（英文版）)

年 卷 期：2018年第32卷第5期

页      面：343-353页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by National Natural Science Foundation of China (NSFC) (81272415 and 81171993) NSFC Key Project (81130046) Guangxi Key Projects (2013GXNSFEA053004) Guangxi Projects (2014GXNSFDA118030) 

主　　题：prostate cancer metastasis NF-κB Bay 11-7082 EMT 

摘      要：Prostate cancer preferentially metastasizes to the bone. However, the underlying molecular mechanisms are still unclear. To explore the effects of a bone-mimicking microenvironment on PC3 prostate cancer cell growth and metastasis, we used osteoblast differentiation medium（ODM; minimal essential medium alpha supplemented with L-ascorbic acid） to mimic the bone microenvironment. PC3 cells grown in ODM underwent epithelial-mesenchymal transition and showed enhanced colony formation, migration, and invasion abilities compared to the cells grown in normal medium. PC3 cells grown in ODM showed enhanced metastasis when injected in mice. A screening of signaling pathways related to invasion and metastasis revealed that the NF-κB pathway was activated, which could be reversed by Bay 11-7082, a NF-κB pathway inhibitor. These results indicate that the cells in different culture conditions manifested significantly different biological behaviors and the NF-κB pathway is a potential therapeutic target for prostate cancer bone metastasis.