Ultrafast photonic PCR

作     者：Jun Ho Son Byungrae Cho SoonGweon Hong Sang Hun Lee Ori Hoxha Amanda J Haack Luke P Lee 

作者机构：Department of BioengineeringUniversity of CaliforniaBerkeleyCA 94720USA Berkeley Sensor and Actuator CenterUniversity of CaliforniaBerkeleyCA 94720USA Department of Electrical Engineering and Computer SciencesUniversity of CaliforniaBerkeleyCA 94720USA Biophysics Graduate ProgramUniversity of CaliforniaBerkeleyCA 94720USA 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2015年第4卷第1期

页      面：267-273页

核心收录：

学科分类：080901[工学-物理电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0803[工学-光学工程] 

基　　金：This work was supported in part by a grant from the Bill&Melinda Gates Foundation(Global Health Grant:OPP1028785) in part by the Global Research Lab Program(2013-050616)through the National Research Foundation of Korea funded by the Ministry of Science,ICT(Information and Communication Technologies)and Future Planning 

主　　题：genomics light-emitting diodes(LEDs) molecular diagnostics personalized medicine plasmonics point-of-care(POC)diagnostics polymerase chain reaction(PCR) 

摘      要：Nucleic acid amplification and quantification via polymerase chain reaction(PCR)is one of the most sensitive and powerful tools for clinical laboratories,precision medicine,personalized medicine,agricultural science,forensic science and environmental *** multiplex PCR,characterized by low power consumption,compact size and simple operation,is ideal for timely diagnosis at the point-of-care(POC).Although several fast/ultrafast PCR methods have been proposed,the use of a simple and robust PCR thermal cycler remains challenging for POC ***,we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon–electron–phonon *** demonstrate an efficient photonic heat converter using a thin gold(Au)film due to its plasmon-assisted high optical absorption(approximately 65%at 450 nm,the peak wavelength of heat source light-emitting diodes(LEDs)).The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150℃ within 3 *** this method,ultrafast thermal cycling(30 cycles;heating and cooling rate of 12.7960.93℃ s^(-1) and 6.660.29℃ s^(-1),respectively)from 55℃(temperature of annealing)to 95℃(temperature of denaturation)is accomplished within 5 *** photonic PCR thermal cycles,we demonstrate here successful nucleic acid(λ-DNA)*** simple,robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures,including on-chip thermal lysis and heating for isothermal amplifications.