MITOCHONDRIAL REDOX IMAGING FOR CANCER DIAGNOSTIC AND THERAPEUTIC STUDIES

作     者：LIN Z.LI HE N.XU MAHSA RANJI SHOKO NIOKA BRITTON CHANCE 

作者机构：Department of RadiologySchool of Medicine University of PennsylvaniaB6 Blockley Hall423 Guardian Drive PhiladelphiaPA 19104-6069USA The Institute for Translational Medicine and Therapeutics School of MedicineUniversity of Pennsylvania PhiladelphiaPA 19104USA Biophotonics Laboratory Department of Electrical Engineering and Computer Science University of Wisconsin-MilwaukeeMilwaukeeWI 53211USA Johnson Research Foundation Department of Biochemistry and Molecular Biophysics School of MedicineUniversity of Pennsylvania 250 Anatomy Chemistry BuildingPhiladelphiaPA 19104USA 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2009年第2卷第4期

页      面：325-341页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the Susan G.Komen Foundation Grant KG081069(PI:L.Z.Li) The Center for Magnietic Resonance and Optical Imaging,and an NIH supported research resource(P41-RR02305,PI:R.Reddy) 

主　　题：Redox ratio reduced nicotinamide adenine dinucleotide NADH flavoprotein flavin adenine dinucleotide FAD calibration 

摘      要：Mitochondrial redox states provide important information about energy-linked biological processes and signaling events in tissues for various disease phenotypes including *** redox scanning method developed at the Chance laboratory about 30 years ago has allowed 3D highresolution(∼50×50×10µm^(3))imaging of mitochondrial redox state in tissue on the basis of the fluorescence of NADH(reduced nicotinamide adenine dinucleotide)and Fp(oxidized flavoproteins including flavin adenine dinucleotide,i.e.,FAD).In this review,we illustrate its basic principles,recent technical developments,and biomedical applications to cancer diagnostic and therapeutic studies in small animal *** developed calibration procedures for the redox imaging using reference standards allow quantification of nominal NADH and Fp concentrations,and the concentration-based redox ratios,e.g.,Fp/(Fp+NADH)and NADH/(Fp+NADH)in *** calibration facilitates the comparison of redox imaging results acquired for different metabolic states at different times and/or with different instrumental settings.A redox imager using a CCD detector has been developed to acquire 3D images faster and with a higher in-plane resolution down to 10µ*** vivo imaging and in vivo imaging of tissue mitochondrial redox status have been demonstrated with the CCD *** of tissue redox imaging in small animal cancer models include metabolic imaging of glioma and myc-induced mouse mammary tumors,predicting the metastatic potentials of human melanoma and breast cancer mouse xenografts,differentiating precancerous and normal tissues,and monitoring the tumor treatment response to photodynamic *** future directions for the development of redox imaging are also discussed.