Combined Effects of Cadmium and Butachlor on Microbial Activities and Community DNA in a Paddy Soil

作     者：WANG Jin-Hua DING Hui LU Yi-Tong SHEN Guo-Qing WANG Jin-Hua1,DING Hui2,LU Yi-Tong2,2 and SHEN Guo-Qing2 1College of Resources & Environment,Shandong Agricultural University,Tai’an 271018(China) 2Department of Environment and Resource,College of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 201101(China)

作者机构：College of Resources & Environment Shandong Agricultural University Tai'an 271018 (China) Department of Environment and Resource College of Agriculture and Biology Shanghai Jiao Tong University Shanghai201101 (China) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2009年第19卷第5期

页      面：623-630页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0904[农学-植物保护] 0703[理学-化学] 070301[理学-无机化学] 

基　　金：supported by the National Key Basic Research Program of China (No.2004CB418503) the National Natural Science Foundation of China (No.40801203) 

主　　题：biomarkers microbial community diversity phosphatase random amplified polymorphic DNA analysis urease 

摘      要：Due to frequent soil Cd contamination and wide use of butachlor in China,there is a need to assess their combined toxicity to soil *** combined effects of cadmium(Cd,10 mg kg-1 soil) and herbicide butachlor(10,50,and 100 mg kg-1 soil) on enzyme activities and microbial community structure in a paddy soil were assessed using the traditional enzyme assays and random amplified polymorphic DNA(RAPD) *** results showed that urease and phosphatase activities were significantly reduced by high butachlor concentration(100 mg kg-1 soil).When the concentrations of Cd and butachlor added were at a ratio of 1:10,urease and phosphatase activities were significantly decreased whereas enzyme activities were greatly improved at the ratio of 1:5,which indicated that the combined effects of Cd and butachlor on soil urease and phosphatase activities depended largely on their addition concentration *** amplified polymorphic DNA(RAPD) analysis showed loss of original bands and appearance of new bands when compared with the control *** amplified polymorphic DNA fingerprints suggested substantial differences between the control and treated soil samples,with apparent changes in the number and size of amplified DNA *** addition of high concentration butachlor and the combined impacts of Cd and butachlor significantly affected the diversity of the microbial *** analysis in conjunction with other biomarkers such as soil enzyme parameters would prove a powerful ecotoxicological *** investigations should be carried out to understand the clear link between RAPD patterns and enzyme activity.