A DUAL-MODALITY OPTICAL BIOPSY APPROACH FOR IN VIVO DETECTION OF PROSTATE CANCER IN RAT MODEL
出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)
年 卷 期：2011年第4卷第3期
页 面：269-277页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：funded in part by Department of Defense(grant#W81XWH-09-1-0406) Texas Ignition Fund
主 题：Prostate cancer light reflectance spectroscopy auto-fluorescence lifetime
摘 要：Ultrasound-guided biopsy procedure for prostate cancer diagnosis,which is the current gold standard,involves quasi-random sampling of prostate tissue without any functional *** this study,we discuss the possibility to augment the detection of prostate cancer using a dual-modality optical approach,which can be coupled with the current needle biopsy *** techniques are light reflectance spectroscopy(LRS)that uses a broadband light source and a CCD array spectrometer,and auto-fluorescence lifetime measurement(AFLM)that uses a custom-designed,time-correlated single photon counting(TCSPC)*** LRS and AFLM were employed sequentially in this study to measure cancer tissue along with control tissue on a rat prostate tumor *** an excitation wavelength of 447 nm,we investigated auto-fluorescence decay curves at the emission wavelengths of 532,562,632 and 684 nm for in vivo and ex vivo *** results show that auto-fluorescence lifetimes at all measured emission wavelengths differ between control and cancerous tissues with 100% specificity and ***,absolute values of hemoglobin derivatives and scattering coe±cient were quantified using in vivo *** part of study also demonstrates that light scattering and absorption are significantly different between the control and cancerous ***,the study demonstrates that both LRS and AFLM may provide several intrinsic biomarkers for in vivo detection of prostate cancer.
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