QUANTITATIVE REDOX SCANNING OF TISSUE SAMPLES USING A CALIBRATION PROCEDURE

作     者：HE N.XU BAOHUA WU SHOKO NIOKA BRITTON CHANCE LIN Z.LI 

作者机构：Molecular Imaging LaboratoryDepartment of Radiology University of PennsylvaniaPhiladelphia PA 19104USA Johnson Research Foundation Department of Biochemistry and Biophysics University of PennsylvaniaPA 19104USA 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2009年第2卷第4期

页      面：375-385页

核心收录：

学科分类：0808[工学-电气工程] 081704[工学-应用化学] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 08[工学] 070303[理学-有机化学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0836[工学-生物工程] 0702[理学-物理学] 

基　　金：the Susan G.Komen Foundation Grant KG081069(PI:L.Z.Li) an NIH supported research resource(P41-RR02305,PI:R.Reddy) the Network of Translational Research in Optical Imaging(NTROI)at the University of Pennsylvania(U54 CA105008,PI:W.S.El-Deiry) an NIH Grant UO1-CA105490(PI:L.A.Chodosh) 

主　　题：NADH flavoprotein redox ratio mitochondria tissue heterogeneity 

摘      要：The fluorescence properties of reduced nicotinamide adenine dinucleotide(NADH)and oxidizedflavoproteins(Fp)including flavin adenine dinucleotide(FAD)in the respiratory chain are sensitive indicators of intracellular metabolic states and have been applied to the studies of mitochondrial function with energy-linked *** redox scanner,a three-dimensional(3D)low temperature imager previously developed by Chance et al.,measures the in vivo metabolicproperties of tissue samples by acquiring fluorescence images of NADH and *** redox ratios,***/(Fp+NADH)and NADH/(Fp+NADH),provided a sensitive index of the mitochondrialredox state and were determined based on relative signal intensity *** we report thefurther development of the redox scanning technique by using a calibration method to quantifythe nominal concentration of the fluorophores in *** redox scanner exhibited very goodlinear response in the range of NADH concentration between 165–1318µM and Fp between90–720µM using snap-frozen solution *** samples such as human tumor mousexenografts and various mouse organs were redox-scanned together with adjacent NADH and Fpstandards of known concentration at liquid nitrogen *** nominal NADH and Fpconcentrations as well as the redox ratios in the tissue samples were quantified by normalizing the tissue NADH and Fp fluorescence signal to that of the snap-frozen solution *** calibration procedure allows comparing redox images obtained at different time,independent of instrument *** quantitative multi-slice redox images revealed heterogeneity inmitochondrial redox state in the tissues.