A novel artificial nerve graft for repairing longdistance sciatic nerve defects:a self-assembling peptide nanofiber scaffold-containing poly (lactic-co-glycolic acid) conduit

作     者：Xianghai Wang Mengjie Pan Jinkun Wen Yinjuan Tang Audra D.Hamilton Yuanyuan Li Changhui Qian Zhongying Liu Wutian Wu Jiasong Guo 

作者机构：Department of Histology and Embryology Southern Medical University Department of Histology and Embryology Xiangnan University Department of Neurology Vanderbilt University Department of Anatomy Li Ka Shing Faculty of Medicine The University of Hong Kong GHM Institute of CNS Regeneration Jinan University Key Laboratory of Tissue Construction and Detection of Guangdong Province Institute of Bone Biology Academy of Orthopedics 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2014年第9卷第24期

页      面：2132-2141页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by a grant from the National Key Basic Research Program of China,No.2014CB542202 and 2014CB542205 the National Natural Science Foundation of China,No.30973095&81371354 a grant from Science and Technology Project of Guangzhou,in China,No.12C32121609 the Natural Science Foundation of Guangdong Province of China,No.S2013010014697 to Guo JS Hong Kong SCI Fund to Wu WT 

主　　题：nerve regeneration peripheral nerve defect artificial nerve graft poly(lactic-co-glycolic acid) self-assembling peptide nanofiber scaffold remyelination axon myelin neuromuscular junction NSFC grants neural regeneration 

摘      要：In this study, we developed a novel artificial nerve graft termed self-assembling peptide nanofiber scaffold （SAPNS）-containing poly（lactic-co-glycolic acid） （PLGA） conduit （SPC） and used it to bridge a 10-mm-long sciatic nerve defect in the rat. Retrograde tracing, behavioral testing and histomorphometric analyses showed that compared with the empty PLGA conduit implantation group, the SPC implantation group had a larger number of growing and extending axons, a markedly increased diameter of regenerated axons and a greater thickness of the myelin sheath in the conduit. Furthermore, there was an increase in the size of the neuromuscular junction and myofiber diameter in the target muscle. These findings suggest that the novel artificial SPC nerve graft can promote axonal regeneration and remyelination in the transected peripheral nerve and can be used for repairing peripheral nerve injury.