Genome Characteristics of the Cyclophragma Undans Nucleopolyhedrovirus: A Distinct Species in Group Ⅰ of Alphabaculovirus

作     者：Zheng Zhu Jun Wang Qianran Wang Feifei Yin Xiaoping Liu Dianhai Hou Lei Zhang Haizhou Liu Jiang Li Basil M. Arif Hualin Wang Fei Deng Zhihong Hu Manli Wang 

作者机构：State Key Laboratory of Virology and China Center for Virus Culture Collection Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China Canadian Forest Service Great Lakes Forestry Centre Sault Ste Marie ON P6A 2E5 Canada 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2018年第33卷第4期

页      面：359-368页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 071007[理学-遗传学] 

基　　金：supported by the National Key R&D Program of China(Grant No.2017YFD0200400) the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB11030400) 

主　　题：染色体分析 核心基因 特征 害虫控制 森林害虫 重复序列 种系发生 进化过程 

摘      要：The Cyclophragma undans nucleopolyhedrovirus(Cyun NPV), a potential pest control agent, was isolated from Cyclophragma undans(Lepidoptera: Lasiocampidae), an important forest pest. In the present study, we performed detailed genome analysis of Cyun NPV and compared its genome to those of other Group Ⅰ alphabaculoviruses. Sequencing of the Cyun NPV genome using the Roche 454 sequencing system generated 142,900 bp with a G + C content of 45%. Genome analysis predicted a total of 147 hypothetical open reading frames comprising 38 baculoviral core genes, 24 lepidopteran baculovirus conserved genes, nine Group Ⅰ Alphabaculovirus conserved genes, 71 common genes, and five genes that are unique to Cyun NPV. In addition, the genome contains 13 homologous repeated sequences(hrs). Phylogenetic analysis groups Cyun NPV under a distinct branch within clade ‘‘a of Group Ⅰ in the genus Alphabaculovirus. Unlike other members of Group Ⅰ, Cyun NPV harbors only nine of the 11 genes previously determined to be specific to Group Ⅰ viruses.Furthermore, the Cyun NPV lacks the tyrosine phosphatase gene and the ac30 gene. The Cyun NPV F-like protein contains two insertions of continuous polar amino acids, one at the conventional fusion peptide and a second insertion at the pretransmembrane domain. The insertions are likely to affect the fusion function and suggest an evolutionary process that led to inactivation of the F-like protein. The above findings imply that Cyun NPV is a distinct species under Group Ⅰ Alphabaculovirus.