SIMULTANEOUS IMAGING OF A lacZ-MARKED TUMOR AND MICROVASCULATURE MORPHOLOGY IN VIVO BY DUAL-WAVELENGTH PHOTOACOUSTIC MICROSCOPY

作     者：LI LI HAO F.ZHANG ROGER J.ZEMP KONSTANTIN MASLOV LIHONG V.WANG 

作者机构：Optical Imaging LaboratoryDepartment of Biomedical Engineering Washington University in St.Louis St.LouisMissouri 63130USA Department of Electrical Engineering University of Wisconsin-Milwaukee MilwaukeeWisconsin 53201USA Department of Electrical and Computer Engineering University of AlbertaEdmontonAlberta T6G2V4Canada 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2008年第1卷第2期

页      面：207-215页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：funded in part by the NIH grants R01 NS46214(BRP)and R01 EB000712 

主　　题：Photoacoustic molecular imaging gene expression reporter gene 

摘      要：Photoacoustic molecular imaging,combined with the reporter-gene technique,can provide a valuable tool for cancer *** expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal,a colorimetric assay for the lacZ-encoded enzymeβ-***-wavelength photoacoustic microscopy was used to non-invasively image the detailed morphology of a lacZ-marked 9L gliosarcoma and its surrounding microvasculature simultaneously in vivo,with a superior resolution on the order of 10μ***-feeding vessels were found,and the expression level of lacZ in tumor was *** future development of new absorption-enhancing reporter-gene systems,we anticipate this strategy can lead to a better understanding of the role of tumor metabolism in cancer initiation,progression,and metastasis,and in its response to therapy.