Effect of Jinlong capsule on proliferation and apoptosis of human pancreatic cancer cells BxPC-3
Effect of Jinlong capsule on proliferation and apoptosis of human pancreatic cancer cells BxPC-3作者机构:Department of OncologyGuang'an men Hospital Affiliated to China Academy of Chinese Medical Sciences Department of Traditional Chinese MedicineFengtai District Community Health Center Jiansheng Pharmaceutical Co.Ltd.
出 版 物:《Journal of Traditional Chinese Medicine》 (中医杂志(英文版))
年 卷 期:2013年第33卷第2期
页 面:205-210页
核心收录:
学科分类:1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
主 题:Pancreatic neoplasms Cell prolifera-tion Apoptosis Jinlong capsule
摘 要:OBJECTIVE:To study the possible roles of Jinlong capsule(JLC) on the proliferation and apoptosis of human pancreatic cancer cells ***:The human pancreatic cancer cells BxPC-3 were treated with JLC at the concentration of 0.05-1.00 mg/mL for 24-120 *** inhibition rate of JLC on human pancreatic cancer cells BxPC-3 was detected by 3-(4,5-dimethiylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) *** cytometry was employed to measure cell apoptosis using Annexin V-FITC/Propidium iodide(AV-FITC/PI) *** cycles were determined by PI *** expression of S100 Calcium binding protein A4(S100A4) in cell matrix was measured by enzyme-linked immunosorbent assay(ELISA).The expression levels of apoptosis-related protein such as BCL2/adenovirus E1B 19 kDa interacting protein 3(BNIP3),B-cell lymphoma/leukemia-2(Bcl-2) and Cys-teinylaspartate specific proteinase 3(Caspase-3) were detected byWestern ***:JLC significantly inhibited the proliferation of human pancreatic cancer cells BxPC-3 in a dose-dependent and time-dependent *** promoted cell apoptosis and maintained cell cycle in S and G 2 /M phase rather than G 1 /G 0 *** expression of S100A4 in the cell matrix was *** expression of cell apoptotic protein BNIP3 was increased while Bcl-2 was ***:JLC can inhibit the proliferation of human pancreatic cancer cells BxPC-3 by stimulating cell apoptosis,arresting the cell cycle at S and G 2 /M phase which blocks the circulation of normal cell cycle and reducing the expression of S100A4 *** pro-apoptosis protein BNIP3 and lower anti-apoptosis protein Bcl-2 levels were found,which may be related to the apoptotic effects of JLC.