Effect of Jinlong capsule on proliferation and apoptosis of human pancreatic cancer cells BxPC-3

作     者：Yaoyuan Li Jieqing Hu Hui Huang Yonghe He 

作者机构：Department of OncologyGuang'an men Hospital Affiliated to China Academy of Chinese Medical Sciences Department of Traditional Chinese MedicineFengtai District Community Health Center Jiansheng Pharmaceutical Co.Ltd. 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2013年第33卷第2期

页      面：205-210页

核心收录：

学科分类：1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：Pancreatic neoplasms Cell prolifera-tion Apoptosis Jinlong capsule 

摘      要：OBJECTIVE:To study the possible roles of Jinlong capsule(JLC) on the proliferation and apoptosis of human pancreatic cancer cells ***:The human pancreatic cancer cells BxPC-3 were treated with JLC at the concentration of 0.05-1.00 mg/mL for 24-120 *** inhibition rate of JLC on human pancreatic cancer cells BxPC-3 was detected by 3-(4,5-dimethiylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) *** cytometry was employed to measure cell apoptosis using Annexin V-FITC/Propidium iodide(AV-FITC/PI) *** cycles were determined by PI *** expression of S100 Calcium binding protein A4(S100A4) in cell matrix was measured by enzyme-linked immunosorbent assay(ELISA).The expression levels of apoptosis-related protein such as BCL2/adenovirus E1B 19 kDa interacting protein 3(BNIP3),B-cell lymphoma/leukemia-2(Bcl-2) and Cys-teinylaspartate specific proteinase 3(Caspase-3) were detected byWestern ***:JLC significantly inhibited the proliferation of human pancreatic cancer cells BxPC-3 in a dose-dependent and time-dependent *** promoted cell apoptosis and maintained cell cycle in S and G 2 /M phase rather than G 1 /G 0 *** expression of S100A4 in the cell matrix was *** expression of cell apoptotic protein BNIP3 was increased while Bcl-2 was ***:JLC can inhibit the proliferation of human pancreatic cancer cells BxPC-3 by stimulating cell apoptosis,arresting the cell cycle at S and G 2 /M phase which blocks the circulation of normal cell cycle and reducing the expression of S100A4 *** pro-apoptosis protein BNIP3 and lower anti-apoptosis protein Bcl-2 levels were found,which may be related to the apoptotic effects of JLC.