A modified chronic ocular hypertension rat model for retinal ganglion cell neuroprotection

作     者：Lichun Zhong 

作者机构：Ocular Science DepartmentToxikon CorporationBedfordMA 01730USA 

出 版 物：《Frontiers of Medicine》 (医学前沿（英文版）)

年 卷 期：2013年第7卷第3期

页      面：367-377页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：organization of foundations 

主　　题：chronic ocular hypertension intraocular pressure retinal ganglion cells neuroprotection glaucoma 

摘      要：This study aimed to modify a chronic ocular hypertension(OHT)rat model to screen for potential compounds to protect retinal ganglion cells(RGCs)from responding to increased intraocular pressure(IOP).A total of 266 rats were prepared and randomly grouped according to different time-points,namely,weeks 3,8,16,and *** were sedated and eye examination was performed to score as the corneal damage on a scale of 1 to *** OHT rat model was created via the injection of a hypertonic saline solution into the episcleral veins once weekly for two *** was identified when the IOP at week 0 was≥6 mmHg than that at week-2 for the same *** RGCs were labeled by injecting 4%*** were sacrificed,and the eyes were enucleated and *** fixed retinas were dissected to prepare flat *** viable RGCs were visualized and *** IOP(meanβ±βSD)was calculated,and data were analyzed by the paired t-test and one-way *** OHT model was created in 234 of 266 rats(87.97%),whereas 32 rats(12.03%)were removed from the study because of the absence of IOP elevation(11.28%)and/or corneal damage scores over 4(0.75%).IOP was elevated by as much as 81.35%for 24 *** average IOP was(16.68β±β0.98)βmmHg in non-OHT eyes(n=234),but was(27.95±0.97)βmmHg in OHT eyes(n=234).Viable RGCs in the OHT eyes were significantly decreased in a time-dependent manner by 29.41%,38.24%,55.32%,and 59.30%at weeks 3,8,16,and 24,respectively,as compared to viable RGCs in the non-OHT eyes(Pβ0.05).The OHT model was successfully created in 88%of the *** IOP in the OHT eyes was elevated by approximately 81%for 24 *** number of viable RGCs was decreased by 59%of the rats in a time-dependent *** modified OHT model may provide an effective and reliable method for screening drugs to protect RGCs from glaucoma.