Diversity of foliar endophytes in wind-fallen Picea abies trees

作     者：Ondřej Koukol Miroslav Kolařík Zuzana Kolářová Petr Baldrian 

作者机构：Department of BotanyFaculty of ScienceCharles University in PragueBenátská212801 Prague 2Czech Republic Laboratory of GeneticsPhysiology and Bioengineering of FungiInstitute of Microbiology of the ASCRv.v.i.Vídeňská108314220 Prague 4Czech Republic Laboratory of Environmental MicrobiologyInstitute of Microbiology of the ASCRv.v.i.Vídeňská108314220 Prague 4Czech Republic 

出 版 物：《Fungal Diversity》 (真菌多样性（英文）)

年 卷 期：2012年第54卷第3期

页      面：69-77页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 0713[理学-生态学] 

基　　金：supported by the Grant Agency of the Czech Republic(project Nr.526/08/0751) the Ministry of Education,Youth and Sports of the Czech Republic(project No.MSM0021620828) the Institutional Research Concept of the Institute of Microbiology of the ASCR,v.v.i.(AV0Z50200510) 

主　　题：Morphospecies ITS rDNA Culturable microfungi Phacidiopycnis Host affinity 

摘      要：The unmanaged Norway spruce montane forests in the Bohemian Forest National Park(Czech Republic)suffered from repeated large-scale bark beetle outbreaks in last *** this study,the diversity of culturable foliar endophyte microfungi in needles originating from eleven recently wind-fallen trees in this area was *** aims were to describe their diversity and to determine the relative host and organ specificities of isolated endophyte species to estimate the species pool and abundance of foliar endophytes before the forest *** were isolated from surface-sterilized needles,and the outgrowing strains were identified based on morphological and molecular characteristics(analyses of ITS1,ITS2 and partial 28S rDNA).Fungal communities in the needles were diverse,with ascomycetes(mostly anamorphs of Helotiales)dominating *** most frequent species(Phacidiopycnis sp.,Cistella acuum,Sirococcus *** two species of Chalara)did not correspond with those recorded in previous studies of Picea *** example,the widely distributed Lophodermium piceae was rarely recorded in this *** pattern may be caused by different methods of sterilization and cultivation or by physiological characteristics of the needles,or it may reflect the species distribution in the studied *** of the Helotiales,along with sequences from GenBank,showed substantial overlap in host affinities,most prominently between Pinus and Picea,and also among species from distant plant lineages.