Redox proteomics of tomato in response to Pseudomonas syringae infection

作     者：Kelly Mayrink Balmant Jennifer Parker Mi-Jeong Yoo Ning Zhu Craig Dufresne Sixue Chen 

作者机构：Department of BiologyGenetics InstituteUniversity of FloridaGainesvilleFLUSA Plant Molecular and Cellular Biology ProgramUniversity of FloridaGainesvilleFLUSA Thermo Fisher Scientific1400 Northpoint ParkwayWest Palm BeachFLUSA Interdisciplinary Center for Biotechnology ResearchUniversity of FloridaGainesvilleFLUSA 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2015年第2卷第1期

页      面：103-114页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0836[工学-生物工程] 0902[农学-园艺学] 0834[工学-风景园林学（可授工学、农学学位）] 090202[农学-蔬菜学] 

基　　金：The redox-proteomics work was partly supported by awards from the National Science Foundation(MCB 0818051 and MCB 1412547)to S.Chen 

主　　题：immunity metabolism modification 

摘      要：Unlike mammals with adaptive immunity,plants rely on their innate immunity based on pattern-triggered immunity(PTI)and effector-triggered immunity(ETI)for pathogen *** oxygen species,known to play crucial roles in PTI and ETI,can perturb cellular redox homeostasis and lead to changes of redox-sensitive proteins through modification of cysteine sulfhydryl *** redox regulation of protein functions has emerged as an important mechanism in several biological processes,little is known about redox proteins and how they function in PTI and *** this study,cysTMT proteomics technology was used to identify similarities and differences of protein redox modifications in tomato resistant(PtoR)and susceptible(prf3)genotypes in response to Pseudomonas syringae pv tomato(Pst)*** addition,the results of the redox changes were compared and corrected with the protein level changes.A total of 90 potential redox-regulated proteins were identified with functions in carbohydrate and energy metabolism,biosynthesis of cysteine,sucrose and brassinosteroid,cell wall biogenesis,polysaccharide/starch biosynthesis,cuticle development,lipid metabolism,proteolysis,tricarboxylic acid cycle,protein targeting to vacuole,and oxidation–*** inventory of previously unknown protein redox switches in tomato pathogen defense lays a foundation for future research toward understanding the biological significance of protein redox modifications in plant defense responses.