Underlying mechanism of ASIC1a involved in acidosis-induced cytotoxicity in rat C6 glioma cells

作     者：Xie-chuan WENG,Jian-quan ZHENG~2,Jin LI,Wen-bin XIAOBeijing Institute of Pharmacology and Toxicology,Beijing 100850,China 

作者机构：Beijing Institute of Pharmacology and Toxicology Beijing China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2007年第28卷第11期

页      面：1731-1736页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：a grant from the National Natural Science Foundation of China(№ 30472019 and 30500620) 

主　　题：acid-sensing ion channel calcium calcium imaging Fluo-3 

摘      要：Aim:To investigate the underlying mechanism of acid-sensing ion channel (ASIC)la involved in the acidosis-induced cytotoxicity of rat C6 glioma ***:The stable ASIC 1 a-silenced C6 cells built with the RNA interference technologywere confirmed by RT-PCR and Western blot *** calcium([Ca2+]i) in both the wild-type rat C6 glioma cells and the ASIC 1a-silenced C6 cellswere analyzed before and after acid application/exposure with the calcium ***:The rapid extracellular pH drop induced the increase of[Ca2+]iin the wild-type C6 cells,but not in the ASIC1a-silenced C6 *** prolonged acid exposure,[Ca2+]iwas lower in the ASIC 1 a-silenced C6 cellsthan that in the control ***:The resultant toxicity of [Ca2+]imightcontribute to the acidosis-induced cytotoxicity.