Detection of oxidative stress and DNA damage in freshwater snail Lymnea leuteola exposed to profenofos

作     者：Daoud Ali Huma Ali Saud Alifiri Saad Alkahtani Abdullah A Alkahtane Shaik Althaf Huasain 

作者机构：Department of ZoologyCollege of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia 2 Department of ChemistryMANITBhopal 462003India 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2018年第12卷第5期

页      面：181-187页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：grateful to the Deanship of Scientific Research at King Saud University for funding this research(RG-1435-076) 

主　　题：Acute toxicity Profenofos ROS oxidative stress DNA damage Lymnea luteola 

摘      要：Extensive production and use of organophosphate pesticide in agriculture,has risen concerned about its ecotoxicity and risk assessment of insecticides,which are more ***,the present investigation was aimed to study the induction of oxidative stress and DNA damage by organophosphate insecticide profenofos(PFF)in freshwater snail Lymnea luteola(***).The median lethal value(96 h LC50)of PFF was estimated as 1.26 mg/L for *** in a semi-static system and on the basis of LC50 value three concentrations viz.,0.126(1/10 of LC50,Sublethal I),0.63(1/2 of LC50,Sublethal II)and 0.84 mg/L(2/3 of LC50,Sublethal III)were *** were exposed to above-mentioned concentrations of PFF along with solvent control(acetone)and negative control for 96 *** haemolymph was collected at 24 and 96 h of after *** heamolymph of PFF exposed snail,lipid peroxide,glutathione reduced glutathione S transferase and superoxide dismutase activities at the tested concentrations significantly differ from those in the *** genotoxicity induced in hemocytes of treated snails was measured by alkaline single cell gel electrophoresis *** data of this experiment demonstrated significantly enhancement of oxidative stress and DNA damage in the treated snails as compared to ***,we observed statistically significant correlations of ROS with DNA damage(%tail DNA)(R^(2)=0.9708)for 24 h and DNA damage(R^(2)=0.9665)for 96 *** of the current experiment can be useful in risk evaluation of PFF among aquatic *** study confirmed the use of comet assay for in vivo laboratory experiments using freshwater snail for selecting the toxic potential of industrial chemicals and environmental contaminants.