Genetic and Physiological Analysis of a Novel Type of Interspecific Hybrid Weakness in Rice

作     者：Chen Chen Hao Chen Jun-Xiang Shan Mei-Zhen Zhu Min Shi Ji-Ping Gao Hong-Xuan Lin 

作者机构：National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai) Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 300 Fenglin Road Shanghai 200032 China 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2013年第6卷第3期

页      面：716-728页

核心收录：

学科分类：08[工学] 09[农学] 0901[农学-作物学] 081201[工学-计算机系统结构] 0812[工学-计算机科学与技术（可授工学、理学学位）] 

基　　金：This work was supported by grants from the National Natural Science Foundation of China (31130071  31121063) and the Ministry of Science and Technology of China (2012AA10A302  2012CB114200).We thank Mr Xiao-Yan Gao and Mr Xiao-Shu Gao for assistance with transmission electron microscopy. No conflict of interest declared 

主　　题：Functional genomics genetics rice. 

摘      要：Hybrid weakness is an important reproductive barrier that hinders genetic exchange between different species at the post-zygotic stage. However, our understanding of the molecular mechanisms underlying hybrid weakness is limited. In this study, we report discovery of a novel interspecific hybrid weakness in a rice chromosome segment substitution line （CSSL） library derived from a cross between the indica variety Teqing （Oryza sativa） and common wild rice （O. rufipogon）. The dominant Hybrid weakness il （Hwil） gene from wild rice is genetically incompatible with Teqing and induced a set of weakness symptoms, including growth suppression, yield decrease, impaired nutrient absorption, and the retardation of crown root initiation. Phytohormone treatment showed that salicylic acid （SA） could restore the height of plants expressing hybrid weakness, while other phytohormones appear to have little effect. Fine mapping indicated that Hwil is located in a tandem leucine-rich repeat receptor-like kinase （LRR-RLK） gene cluster. Within the 13.2-kb candidate region on the short arm of chromosome 11, there are two annotated LRR-RLK genes, LOC_Osllg07230 and LOC_Osllg07240. The Teqing allele of LOCOsllg07230 and the wild rice allele of LOC_Osllg07240 encode predicted functional proteins. Based on the genetic inheritance of hybrid weakness, LOC_Osllg07240 is implicated as the candidate gene for Hwil. Functional analysis of Hwil will expand our knowledge of the regulation of hybrid weakness in rice.