Effects of dichloroacetate on the activation of the mitochondrial pathway in C6 cells in vitro

作     者：赵欣 王欣 余克富 段瑀 李捷思 赵炳祥 张烜 张强 

作者机构：北京大学医学部药学院药剂学系北京100191 北京大学医学部天然药物及仿生药物国家重点实验室北京100191 

出 版 物：《Journal of Chinese Pharmaceutical Sciences》 (中国药学（英文版）)

年 卷 期：2011年第20卷第5期

页      面：460-465页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：National Natural Science Foundation of China (Grant No.30873170) the National Basic Research Program of China(973 Program 2007CB935800 and 2009CB930300) 

主　　题：Mitochondria metabolism Dichloroacetate C6 glioma Pyruvate dehydrogenase Reactive oxygen species Mitochondrial membrane potential 

摘      要：Mitochondria are increasingly recognized as important targets for tumor treatment because of their central roles in apoptotic pathways and cellular metabolism. Dichloroacetate （DCA）, a low molecular weight mitochondria-targeting agent, exhibits potential therapeutic effects for tumors. Based on the effects of DCA on tumor cellular metabolism, we carried out this study to investigate the anti-tumor activity of DCA in C6 glioma cells in vitro. The results showed that DCA was able to increase the activity of pyruvate dehydrogenase （PDH）, induce the production of reactive oxygen species （ROS） and reduce the mitochondrial membrane potential （MMP） in C6 ceils in vitro （P〈0.05 or 0.01）, indicating that the anti-tumor effects of DCA in C6 cells could be through the activation of the mitochondrial pathway. In conclusion, mitochondria could be a viable therapeutic target for the treatment of glioma.