Effects of All－trans Retinoic Acid on hTERT Gene Expression and Telomerase Activity of HL－60 Cells

作     者：HEDongmei ZHANGYuan He Dongmei;ZHANG Yuan

作者机构：InstituteofHematologyMedicalCollegeofJinanUniversityCuangzhou510632China 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2003年第2卷第3期

页      面：169-171,192页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work supported by the Guangdong Provincial Key Foundation of science and Technology Program(99M01204G) Guangzhou City Key Foundation of science and Technology Program(2001-Z-037-01) 

主　　题：hTERT基因 端粒转移酶 HL-60细胞 白血病 全反式维甲酸 

摘      要：Objective: To investigate the effects of all-trans retinoic acid (ATRA) on human telomerase reverse transcriptase (hTERT) protein expression and telomerase activity in HL-60 cells. Methods: The expression of hTERT protein was assayed by immunofluorescence using fluoresce isothiocyanate label and telomerase activity was determined by polymerase chain reaction enzyme-linked immunoassay with HL-60 cells untreated or treated with ATRA. Cell cycle was analyzed by flow cytometry. Results: After treatment with 1μmol/L ATRA for 24, 48, 72 h, mean fluorescence intensity of hTERT protein in HL-60 cells was 61.87±4.36, 37.47±2.85， 33.45±2.37，respectively. There was a significant decrease in hTERT protein expression compared to the cells untreated, and the effect had statistically significant difference (P0.05).Telomerase activity was decreased significantly in HL-60 cells treated with 1μmol/L ATRA for 48, 72h as compared to the cells untreated (P0.05). Conclusion: ATRA could inhibit telomerase activity and hTERT gene expression in HL-60 cells.