Effects of six compounds with different chemical structures on melanogenesis

作     者：Rakotomalala Manda Heriniaina DONG Jing Praveen Kumar Kalavagunta WU Hua-Li YAN Dong-Sheng SHANG Jing 

作者机构：State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjing 210009China Jiangsu Key Laboratory of TCM Evaluation and Translational ResearchChina Pharmaceutical UniversityNanjing 211198China School of Ophthalmology and OptometryEye HospitalWenzhou Medical UniversityWenzhou 325035China State Key Laboratory of OphthalmologyOptometry and Visual ScienceWenzhou 325035China 

出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)

年 卷 期：2018年第16卷第10期

页      面：766-773页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 100706[医学-药理学] 1002[医学-临床医学] 0703[理学-化学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.81874331) the Open Project of State Key Laboratory of Natural Medicines(No.3144060130) 

主　　题：Vitiligo Melanogenesis Tyrosinase MITF Zebrafish 

摘      要：Several chemical compounds can restore pigmentation in vitiligo through mechanisms that vary according to disease *** the present study,we investigated the melanogenic activity of six structurally distinct compounds,namely,scopoletin,kaempferol,chrysin,vitamin D_3,piperine,and *** determined their effectiveness,toxicity,and mechanism of action for stimulating pigmentation in B16F10 melanoma cells and in a zebrafish *** melanogenic activity of 6-benzylaminopurine,the compound identified as the most potent,was further verified by measuring green fluorescent protein concentration in tyrp1 a:eGFP(tyrosinase-related protein 1)zebrafish and mitfa:eGFP(microphthalmia associated transcription factor)zebrafish and antioxidative *** the tested compounds were found to enhance melanogenesis responses both in vivo and in vitro at their respective optimal concentration by increasing melanin content and expression of TYR and MITF.6-Benzyamino-purine showed the strongest re-pigmentation action at a concentration of 20μmol·L^(-1 )in vivo and 100μmol·L^(-1) in vitro,and up-regulated the strong fluorescence expression of green fluorescent protein in tyrp1 a:e GFP and mitfa:e GFP zebrafish in ***,its relative anti-oxidative activity was found to be very ***,our results indicated that 6-benzylaminopurine stimulated pigmentation through a direct mechanism,by increasing melanin content via positive regulation of tyrosinase activity in vitro,as well as up-regulating the expression of the green fluorescent protein in transgenic zebrafish in vivo.