Rat model for study of the essence of spleen deficiency and dampness in Chinese medicine

作     者：Xiao-Chun Han Xu-Ming Ji Lin Liu Shuang Zhang Li Sun Qiu-Jian Feng Shi-Jun Wang 

作者机构：College of Traditional Chinese MedicineShandong University of Traditional Chinese MedicineJinanChina Department of Special ExaminationAffiliated Hospital of Shandong University of Traditional Chinese MedicineJinanChina 

出 版 物：《Traditional Medicine Research》 (TMR传统医学研究)

年 卷 期：2018年第3卷第5期

页      面：251-257页

学科分类：10[医学] 

基　　金：National Natural Science Foundation of China(NO.81703838) National Key Basic Research and Development Program(973 Program)funded project,spleen and dampness of traditional Chinese medicine drug research(NO.2013CB531803) Shandong Province,Chinese medicine science and technology development project,project number:2015-008 Shandong Province,Chinese medicine science and technology development project,project number:2017-027 

主　　题：Spleen deficiency Dampness Animal model 

摘      要：Objective:To explore the essence of spleen deficiency and dampness in Chinese medicine by successfully constructing a rat model with this ***:Rat models with the syndrome of dampness and spleen deficiency were established with the use of a high-fat,low-protein diet and excessive fatigue induced by loaded swimming.A total of 25 common clinical serological markers were *** T test,rank test,and partial least squares regression-discriminant analysis were used to analyze the ***:Total protein,albumin,motilin,interferon-γ,interleukin-2,immunoglobulin A,immunoglobulin G,and complement 3 levels in the model rats were lower than those in the control group(P=0.029,P=0.032,P0.001,P0.001,P0.001,P0.001,P0.001,P0.001,respectively).The serum creatinine,total cholesterol,aldosterone,antidiuretic hormone,gastrin,interleukin-4,somatostatin,atrial natriuretic peptide,and vasoactive intestinal peptide levels in the model group were higher than those in the control group(P0.001,P=0.015,P0.001,P0.001,P0.001,P0.001,P0.001,P0.001,P0.001,respectively).There was no statistical difference between the two groups in aspartate transaminase,alanine aminotransferase,globulin,albumin/globulin,blood urea nitrogen,high-density lipoprotein,and low-density *** model animal syndrome used two important variables in analysis with partial least squares regression-discriminant *** the same time,the two dimensions were difficult to distinguish for each biological ***:Spleen deficiency is often associated with *** index that represents spleen deficiency can also represent ***,immunoglobulins,and gastrointestinal hormones play a major causative role in both spleen deficiency and dampness.