Discoursing on Soxhlet extraction of ginseng using association analysis and scanning electron microscopy

作     者：Yuan Yue Zi-Dong Qiu Xian-You Qu Ai-Ping Deng Yuan Yuan Lu-Qi Huang Chang-Jiang-Sheng Lai 

作者机构：Chongqing Academy of Chinese Materia MedicaChongqing Key Laboratory of Traditional Chinese Medicine ResourcesChongqing Sub-center of National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesChongqing 400065PR China National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesState Key Laboratory Breeding Base of Dao-di HerbsBeijing 100700PR China State Key Laboratory of Natural Medicines(China Pharmaceutical University)No.24 Tongjia laneNanjing 210009PR China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2018年第8卷第5期

页      面：312-317页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：financial support of the key project at the central government level:The Ability Establishment of Sustainable Use for Valuable Chinese Medicine Resources(No.2060302) the Independent Research Grant of National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences(No.ZZXT201608) the National Natural Science Foundation of China(No.81603293) 

主　　题：Ginseng Ginsenosides Soxhlet extraction Repeatability Siphon times 

摘      要：Soxhlet extraction is a common method of sample preparation. However, there has been no discussion about the efficiency of Soxhlet extraction from different batches and the factors that cause content fluctuation. In this study, Panax ginseng was selected as a model sample. Soxhlet extraction by means of a water bath, which has always been neglected, was identified as a novel key factor in the poor repeat- ability in different batches of Soxhlet extraction, as it can affect the siphon times and reflux time, which have been positively correlated with the ginsenoside contents. By substituting round bottom flasks in the same column, the relative standard deviation of the most fluctuated compound, ginsenoside Rbl, was decreased from 24.6% to 5.02%. Scanning electron microscopy analysis confirmed that the breakdown of the surface of the ginseng powder in the Soxhlet extraction led to a better dissolution of ginsenosides, indicating that chloroform may promote the extraction of ginsenosides by disrupting the cell structure. Moreover, 70% methanol was regarded as the better solvent for extracting the ginsenosides. Overall, this work offers a practical and effective protocol for improving the accuracy and repeatability of Soxhlet extraction methodology for ginsenosides and other analytes.