Bacterial Community and Function of Biological Activated Carbon Filter in Drinking Water Treatment

作     者：ZHANG DuoYing LI WeiGuang ZHANG ShuMei LIU Miao ZHAO XiaoYu ZHANG XianCheng 

作者机构：School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin 150090 Heilongjiang China National Engineering Research Centre of Urban Water Resources Harbin 150090 Heilongjiang China Institute of Microbiology of Heilongjiang Academy of Sciences Harbin 150010 Heilongjiang China Heilongjiang East University Harbin 150086 Heilongjiang China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2011年第24卷第2期

页      面：122-131页

核心收录：

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 071007[理学-遗传学] 0713[理学-生态学] 

基　　金：supported by grants from National Natural Science Foundation of China (50678046) Heilongjiang Provincial Natural Science Foundation for Distinguished Young Scholars (JC200708) 

主　　题：Biological activated carbon Bacterial community Denaturing gradient gel electrophoresis 

摘      要：Objective It aims to investigate the changes in composition and structure of bacterial communities de-veloping on biological activated carbon （BAC） particles, and the bacterial functions. Method A pilot plant had been in service for 180 days, aiming to develop bacterial communities on acti-vated carbon naturally. After 180 days of operation, the bacterial communities were determined by dena-turing gradient gel electrophoresis （DGGE） analyses of PCR-amplified 16S rRNA genes. The study on community composition and the phylogenetic relationships of the organisms was complemented by a se-quence analysis of cloned PCR products from 16S rRNA genes. Gas chromatorgaphy-mass （GC-MS） mea-surement was used to determine organic chemical composition of inflow and outflow water on the 300th day. TOC and NH 4 ＋ -N were also tested in this experiment. Results It showed that the stable bacterial structure did not develop on BAC particles until the 9th month during running time of the BAC filter. The communities were finally dominated by Pseudomonas sp., Ba-cillus sp., Nitrospira sp., and an uncultured bacterium. Stable bacterial communities played an important role in removal of NH 4 ＋ -N and total organic carbon （TOC）. Results from gas chromatorgaphy-mass （GC-MS） showed that 36 kinds of chemicals in feed water were eliminated, and concentrations of 5 kinds of chemicals decreased. These chemicals served as nutrients for the dominant bacteria. Conclusion The findings from the study suggested that the stability of microbial structure was beneficial for improving NH 4 ＋ -N and TOC removal efficiencies. The dominant bacteria had the advantage of biode-grading a wide range of organic chemicals and NH 4 ＋ -N.